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B1MHC6 (MEND_MYCA9) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Short name=SEPHCHC synthase
EC=2.2.1.9
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene names
Name:menD
Ordered Locus Names:MAB_3933c
OrganismMycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]
Taxonomic identifier561007 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium abscessus

Protein attributes

Sequence length540 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity. HAMAP-Rule MF_01659

Catalytic activity

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2. HAMAP-Rule MF_01659

Cofactor

Magnesium or manganese By similarity. HAMAP-Rule MF_01659

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8. HAMAP-Rule MF_01659

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01659

Sequence similarities

Belongs to the TPP enzyme family. MenD subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5405402-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase HAMAP-Rule MF_01659
PRO_1000187080

Sequences

Sequence LengthMass (Da)Tools
B1MHC6 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 2CF599BE49C16D66

FASTA54056,592
        10         20         30         40         50         60 
MNPSTAQAHA VVDELIRGGV RDVVLCPGSR NAPLAFALHD ADKAGRLRLH VRIDERTAGF 

        70         80         90        100        110        120 
LAVGLAAASG SPVPVAMTSG TAVANLGPAV VEANYARVPL IVLSANRPYE LLGTGANQTM 

       130        140        150        160        170        180 
EQLGYFGTQV RAAISIGLAE EGSEKIEAQN PHWRSATCRV LAAAKGSRTA NAGPVQFDIP 

       190        200        210        220        230        240 
LREPLVPDRD GGPIPAGRPG GAPWTYTPDV TFDEPVEIDL TADTVVIAGH GAATHPNLSM 

       250        260        270        280        290        300 
LPTVAEPTAP QPDNPVHPLA LTLLHPQQVI MLGRPTLHRQ VSSLLADSRI PVYALTTGPR 

       310        320        330        340        350        360 
WPDVSGNSQA TGTRAVTSGS PDPAWLQRCA HAHHKALDAV RGQLSTHPLT TGLHVAAHVC 

       370        380        390        400        410        420 
GSLRDGDQLV LGASNPVRDA ALVPWGAKDV RVRSNRGVAG IDGTVSTAIG AALAHRGGRT 

       430        440        450        460        470        480 
VALIGDLTFV HDSSGLLIGP TEPRPENLTI VVSNDNGGGI FELLEQGDPR FHDVSSRIFG 

       490        500        510        520        530        540 
TPHDVDIAAL CRAYHVDSQS ISVEELGPAL DEPARGIRVL EVKADRSTLR ELHAAIRAAL 

« Hide

References

[1]"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus."
Genoscope
Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19977 / DSM 44196.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU458896 Genomic DNA. Translation: CAM64007.1.
RefSeqYP_001704661.1. NC_010397.1.

3D structure databases

ProteinModelPortalB1MHC6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING561007.MAB_3933c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAM64007; CAM64007; MAB_3933c.
GeneID5966396.
KEGGmab:MAB_3933c.
PATRIC17979682. VBIMycAbs55940_4006.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1165.
HOGENOMHOG000218359.
KOK02551.
OMAWRSAVCR.
OrthoDBEOG6NWBQW.
ProtClustDBPRK07449.

Enzyme and pathway databases

BioCycMABS561007:GJTG-3953-MONOMER.
UniPathwayUPA00079; UER00164.

Family and domain databases

HAMAPMF_01659. MenD.
InterProIPR004433. MenaQ_synth_MenD.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamPF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFPIRSF004983. MenD. 1 hit.
TIGRFAMsTIGR00173. menD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMEND_MYCA9
AccessionPrimary (citable) accession number: B1MHC6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 29, 2008
Last modified: February 19, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways