B1MGQ4 (B1MGQ4_MYCA9) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

MAB_0489

Organism

Mycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]

Taxonomic identifier

561007 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length	930 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B1MGQ4 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: DF8407830BA52FF5	FASTA	930	101,785
	10 20 30 40 50 60 MRRLVIVESP TKARKIAGYL GDGYVVESSR GHIRDLPRAA ADVPAKYKSE PWARLGVNVD 70 80 90 100 110 120 ADFEPLYIVS PDKKGTVTEL KGLLKDVDEL YLATDGDREG EAIAWHLLET LKPKVPVRRM 130 140 150 160 170 180 VFHEITEQAI LAAAQDPRDL DNNLVDAQET RRILDRLYGY EVSPVLWKKV GRNLSAGRVQ 190 200 210 220 230 240 SVATRIIVQR ERDRMAFRSA GYWDLGAELD AGKDNPAAKP PRFHARLVSV DALRVASGRD 250 260 270 280 290 300 FDSLGQVKET SNGPGVLVLD ETRANALVAG LSQATLTVVS AEEKPYTRKP YPPFMTSTLQ 310 320 330 340 350 360 QEAGRKLRFS SERTMSIAQR LYENGYITYM RTDSTTLSDS ALQAARSQAA ELYGNEYVHP 370 380 390 400 410 420 SPRQYTRKVK NAQEAHEAIR PAGETFKTPG QLHSSLDNDE FRLYELIWQR TVASQMADAR 430 440 450 460 470 480 GTTLSLRLAG TATSGEQVVF TASGRTITFP GFLSAYVETV DELSGGEADD EERRLPQLRQ 490 500 510 520 530 540 GQSVTVAELS ADGHTTNPPA RYTEASLIKA LEELGIGRPS TYSSIIRTIQ DRGYVHKRGS 550 560 570 580 590 600 ALVPSWTAFA VIGLLEQHFG RLVDYDFTAA MEDDLDEIAS GNERRTNWLN AFYFGGDHGV 610 620 630 640 650 660 EGSVARAGGL KRLVGVNLEE IDAREINSIK LFDDDQGRPI YVRVGKNGPY LERMVADPDG 670 680 690 700 710 720 EAGALIPQRA NVKDGVTPDE LTVEMAEQLF AIPQEGRTLG VDPETGHEIV AKDGRFGPYV 730 740 750 760 770 780 TEVLPAPPEP VEAEKPKRGA KKVEGPKPRT GSLLKSMTLE TVTLEDALRL LSLPRVVGVD 790 800 810 820 830 840 PASGEEITAQ NGRYGPYLKR GTDSRSLATE GQIFTVTLDE ALKIYSEPKR RGGQAASAAP 850 860 870 880 890 900 LRELGVDSAT GKPMVIKDGR FGPYVTDGET NASLRKGDDV LSITDERASE LLADRRARGP 910 920 930 VKRAKKAPAK AAKKAPAKKA PAKKAAKKAT

References

[1]

"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus." Genoscope Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU458896 Genomic DNA. Translation: CAM60588.1.
RefSeq	YP_001701242.1. NC_010397.1.
3D structure databases
ProteinModelPortal	B1MGQ4.
ModBase	Search...
Protein-protein interaction databases
STRING	B1MGQ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000007812; EBMYCP00000007751; EBMYCG00000007810.
GeneID	5963026.
GenomeReviews	Gene locus MAB_0489 in contig CU458896_GR.
KEGG	mab:MAB_0489.
PATRIC	17972647. VBIMycAbs55940_0528.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016895.
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1MGQ4_MYCA9

Accession

Primary (citable) accession number: B1MGQ4

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)