B1MGE7 (RL2_MYCA9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L2

Gene names

Name:	rplB
Ordered Locus Names:	MAB_3817c

Organism

Mycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]

Taxonomic identifier

561007 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome By similarity. HAMAP MF_01320_B
Subunit structure	Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome By similarity.
Sequence similarities	Belongs to the ribosomal protein L2P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	50S ribosomal protein L2 HAMAP MF_01320_B		PRO_1000141583

Sequences

	Sequence		Length	Mass (Da)
	B1MGE7 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 11AE52D5640C8988	FASTA	278	30,251
	10 20 30 40 50 60 MAIRKYKPTT PGRRGSSVAD FSEITRSTPE KSLIRPLHGT GGRNAHGRIT TRHKGGGHKR 70 80 90 100 110 120 AYRLIDFRRH DKDGVNAKVA HIEYDPNRTA RIALLHFLDG EKRYIIAPVG LSQGDVVESG 130 140 150 160 170 180 ANADIKPGNN LPLRNIPTGT TVHAVELRPG GGAKLARSAG SGIQLLGKEG AYASLRMPSG 190 200 210 220 230 240 EIRRVDVRCR ATVGEVGNAE QANINWGKAG RMRWKGKRPT VRGVVMNPVD HPHGGGEGKT 250 260 270 SGGRHPVSPW GKPEGRTRKP NKASDKLIVR RRRTGKKR

References

[1]

"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus." Genoscope Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19977 / DSM 44196.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU458896 Genomic DNA. Translation: CAM63892.1.
RefSeq	YP_001704546.1. NC_010397.1.
3D structure databases
ProteinModelPortal	B1MGE7.
SMR	B1MGE7. Positions 2-271.
ModBase	Search...
Protein-protein interaction databases
STRING	B1MGE7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000004096; EBMYCP00000004035; EBMYCG00000004094.
GeneID	5967469.
GenomeReviews	Gene locus MAB_3817c in contig CU458896_GR.
KEGG	mab:MAB_3817c.
PATRIC	17979438. VBIMycAbs55940_3888.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016403.
HOGENOM	HBG748739.
OMA	QSNINWG.
ProtClustDB	PRK09374.
Enzyme and pathway databases
BioCyc	MABS561007:MAB_3817C-MONOMER.
Family and domain databases
HAMAP	MF_01320_B. Ribosomal_L2_B. [Tree]
InterPro	IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR022666. Rbsml_prot_L2_RNA-bd_dom. IPR002171. Ribosomal_L2. IPR014726. Ribosomal_L2_3. IPR005880. Ribosomal_L2_bac-type. IPR022669. Ribosomal_L2_C. IPR022671. Ribosomal_L2_CS. IPR014722. Transl_SH3-like_sub. IPR008991. Translation_prot_SH3-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:2.30.30.30. Ribosomal_L2. 1 hit. G3DSA:4.10.950.10. Ribosomal_L2. 1 hit.
KO	K02886.
PANTHER	PTHR13691:SF5. Ribosom_L2_bac. 1 hit. PTHR13691. Ribosomal_L2. 1 hit.
Pfam	PF00181. Ribosomal_L2. 1 hit. PF03947. Ribosomal_L2_C. 1 hit. [Graphical view]
PIRSF	PIRSF002158. Ribosomal_L2. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF50104. Transl_SH3_like. 1 hit.
TIGRFAMs	TIGR01171. RplB_bact. 1 hit.
PROSITE	PS00467. RIBOSOMAL_L2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RL2_MYCA9

Accession

Primary (citable) accession number: B1MGE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program