B1ME58 (B1ME58_MYCA9) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A HAMAP MF_01897
EC=5.99.1.3 HAMAP MF_01897

Gene names

Name:	gyrA HAMAP MF_01897
Ordered Locus Names:	MAB_0019

Organism

Mycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]

Taxonomic identifier

561007 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length	839 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. SAAS SAAS006691 HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. SAAS SAAS006691 HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity. HAMAP MF_01897
Subcellular location	Cytoplasm By similarity SAAS SAAS006691 HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP MF_01897

Ontologies

Keywords
Cellular component	Cytoplasm SAAS SAAS006691 HAMAP MF_01897
Ligand	ATP-binding SAAS SAAS006691 HAMAP MF_01897 DNA-binding SAAS SAAS006691 HAMAP MF_01897 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS006691 HAMAP MF_01897
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B1ME58 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 90C0CE778D2D75C1	FASTA	839	92,311
	10 20 30 40 50 60 MTDTTLPPGG DDAVDRIEPV DIQQEMQRSY IDYAMSVIVG RALPEVRDGL KPVHRRVLYA 70 80 90 100 110 120 MYDSGFRPDR SHAKSARSVA ETMGNYHPHG DASIYDTLVR MAQPWSLRYP LVDGQGNFGS 130 140 150 160 170 180 PGNDPAAAMR YTEARLTPLA MEMLREIDEE TVDFIPNYDG RVMEPTVLPS RFPNLLANGS 190 200 210 220 230 240 GGIAVGMATN MPPHNLRELA EAVYWALDNH EADEETTLKA VCEKITGPDF PTSGLIVGTQ 250 260 270 280 290 300 GIHDAYTTGR GSIRMRGVAE IEEDSKGRTS LVITELPYQV NHDNFITSIA EQVRDGKIAG 310 320 330 340 350 360 ISNIEDQSSD RVGLRIVVVL KRDAVAKVVL NNLYKHTQLQ TSFGANMLSI VDGVPRTLRL 370 380 390 400 410 420 DQLIRLYVNH QLDVIIRRTR YRLRKANERA HILRGLVKAL DALDEVIALI RASQTVDIAR 430 440 450 460 470 480 TGLIELLDVD EIQAQAILDM QLRRLAALER QKIIDDLAKI EAEIADLEDI LAKPERQRAI 490 500 510 520 530 540 VKDELAEITE KYGDDRRTRI ISADGDVADE DLIAREDVVV TITETGYAKR TKTDLYRSQK 550 560 570 580 590 600 RGGKGVQGAG LKQDDIVKHF FVCSTHDWIL FFTTKGRVYR AKAYDLPEAA RTARGQHVAN 610 620 630 640 650 660 LLAFQPEERI AQVIQIKSYE DAPYLVLATK NGLVKKSKLT EFDSNRSGGL VAVNLRDGDE 670 680 690 700 710 720 LVGAVLCSAE DDLLLVSAHG QSIRFSATDE ALRPMGRATS GVQGMRFNGE DDLLSLNVVR 730 740 750 760 770 780 EGTYLLVATS GGYSKRTAIE EYPVQGRGGK GVLTVQYDPR RGSLVGALVV DEESELYAIT 790 800 810 820 830 SGGGVIRTIA KQVRKAGRQT KGVRLMNLGE GDTLLAIAHN ADEGDADPDE DAAGTTAGE

References

[1]

"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus." Genoscope Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU458896 Genomic DNA. Translation: CAM60122.1.
RefSeq	YP_001700776.1. NC_010397.1.
3D structure databases
ProteinModelPortal	B1ME58.
ModBase	Search...
Protein-protein interaction databases
STRING	B1ME58.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000008085; EBMYCP00000008024; EBMYCG00000008083.
GeneID	5962565.
GenomeReviews	Gene locus MAB_0019 in contig CU458896_GR.
KEGG	mab:MAB_0019.
PATRIC	17971679. VBIMycAbs55940_0053.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016653.
HOGENOM	HBG635183.
OMA	TGRGRIY.
ProtClustDB	PRK05560.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B1ME58_MYCA9

Accession

Primary (citable) accession number: B1ME58

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)