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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Mycobacterium abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei80Charge relay systemUniRule annotation1
Active sitei155Charge relay systemUniRule annotation1
Active sitei179Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMABS36809:G12XF-3357-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:MAB_3341c
OrganismiMycobacterium abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543)
Taxonomic identifieri561007 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium abscessus
Proteomesi
  • UP000007137 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000951511 – 492Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST492

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi561007.MAB_3341c.

Structurei

3D structure databases

ProteinModelPortaliB1MDU8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
OMAiKEYFGAG.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1MDU8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDLIRQDAA ALAALIHSGE VSSVEVTRAH LDQIASTDET YRAFLHVAGE
60 70 80 90 100
QALAAAKDVD DAIAAGSVPS GLAGVPLALK DVFTTRDMPT TCGSKILENW
110 120 130 140 150
VPPYDATVTA RLRGAGIPIL GKTNMDEFAM GSSTENSAYG PTRNPWDVER
160 170 180 190 200
VPGGSGGGSA AALAAFQAPL AIGTDTGGSI RQPAALTATV GVKPTYGTVS
210 220 230 240 250
RFGLVACASS LDQGGPCART VLDTALLHQV IAGHDPRDST SVDEPVPDVV
260 270 280 290 300
AAARAGASGD LKGVRVGVVT QLRGDGYQPG VMASFDAAVE QLTALGADVV
310 320 330 340 350
EVSCPHFEYA LPAYYLILPS EVSSNLARFD AMRYGMRVGD DGTHSAEEVM
360 370 380 390 400
ALTRAAGFGP EVKRRIMIGT YALSAGYYDA YYGRAQKVRT LIKRDLERAY
410 420 430 440 450
EQVDVLVTPA TPTTAFRLGE KVDDPLAMYQ FDLCTLPLNL AGHCGMSVPS
460 470 480 490
GLSADDGLPV GLQIMAPALA DDRLYRVGAA YETARGALPA AL
Length:492
Mass (Da):51,323
Last modified:April 29, 2008 - v1
Checksum:iA35D9234EF8C0FA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU458896 Genomic DNA. Translation: CAM63417.1.

Genome annotation databases

EnsemblBacteriaiCAM63417; CAM63417; MAB_3341c.
PATRICi17978464. VBIMycAbs55940_3403.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU458896 Genomic DNA. Translation: CAM63417.1.

3D structure databases

ProteinModelPortaliB1MDU8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi561007.MAB_3341c.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM63417; CAM63417; MAB_3341c.
PATRICi17978464. VBIMycAbs55940_3403.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
OMAiKEYFGAG.

Enzyme and pathway databases

BioCyciMABS36809:G12XF-3357-MONOMER.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_MYCA9
AccessioniPrimary (citable) accession number: B1MDU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.