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B1MDI3 (B1MDI3_MYCA9) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
EC=2.1.1.228 HAMAP MF_00605
Alternative name(s):
M1G-methyltransferase HAMAP MF_00605
tRNA [GM37] methyltransferase HAMAP MF_00605
Gene names
Name:trmD HAMAP MF_00605
Ordered Locus Names:MAB_3226c
OrganismMycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]
Taxonomic identifier561007 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length242 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605 RuleBase RU003464

Catalytic activity

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605

S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. RuleBase RU003464

Subunit structure

Homodimer By similarity. HAMAP MF_00605 RuleBase RU003464

Subcellular location

Cytoplasm By similarity HAMAP MF_00605 RuleBase RU003464.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family. HAMAP MF_00605 RuleBase RU003464

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region133 – 1386S-adenosyl-L-methionine binding By similarity HAMAP MF_00605

Sites

Binding site1091S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP MF_00605

Sequences

Sequence LengthMass (Da)Tools
B1MDI3 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 007C32D438239CB2

FASTA24226,263
        10         20         30         40         50         60 
MKIDVVTIFP EYLQPVRQSL PGKAIDAGLV DVAVHDLRRW THDVHKSVDD SPYGGGPGMV 

        70         80         90        100        110        120 
MKPTVWGDAL DEICTSETLL VVPTPAGYPF TQETAWQWST EDHLVIACGR YEGIDQRVAD 

       130        140        150        160        170        180 
DAATRMRVRE VSIGDYVLNG GEAAALVIIE AVLRLVPGVL GNALSAQEDS HSEGMASLLE 

       190        200        210        220        230        240 
GPSYTRPPSW RGMDVPPVLL SGDHAKIAAW RAEQSRQRTI ERRPDLLGFD SPTGEHGGDG 


LS

« Hide

References

« Hide 'large scale' references
[1]"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus."
Genoscope
Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Crystal structure of a tRNA-guanine-N1-methyltransferase from Mycobacterium abscessus."
Seattle Structural Genomics Center for Infectious Disease (SSGCID)
Edwards T.E., Gardberg A.S., Sankaran B.
Submitted (FEB-2011) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU458896 Genomic DNA. Translation: CAM63302.1.
RefSeqYP_001703956.1. NC_010397.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3QUVX-ray1.70A/B1-242[»]
ProteinModelPortalB1MDI3.
ModBaseSearch...

Protein-protein interaction databases

STRINGB1MDI3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000005930; EBMYCP00000005869; EBMYCG00000005928.
GeneID5965728.
GenomeReviewsGene locus MAB_3226c in contig CU458896_GR.
KEGGmab:MAB_3226c.
PATRIC17978222. VBIMycAbs55940_3284.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000016420.
HOGENOMHBG285805.
OMAVCGRFEG.

Family and domain databases

HAMAPMF_00605. TrmD.
[Tree]
InterProIPR016009. tRNA_m1G_MeTrfase.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
[Graphical view]
Gene3DG3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KOK00554.
PANTHERPTHR10056. PTHR10056. 1 hit.
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMsTIGR00088. TrmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB1MDI3_MYCA9
AccessionPrimary (citable) accession number: B1MDI3
Entry history
Integrated into UniProtKB/TrEMBL: April 29, 2008
Last sequence update: April 29, 2008
Last modified: December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info