B1MD96 (SYP_MYCA9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	MAB_3140c

Organism

Mycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]

Taxonomic identifier

561007 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	Proline--tRNA ligase HAMAP MF_01569		PRO_1000199406

Sequences

	Sequence		Length	Mass (Da)
	B1MD96 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: A72925B170DBD1F6	FASTA	580	62,998
	10 20 30 40 50 60 MITRLSELFV RTLRDDPADA EVPSHKLLIR AGYVRPVAPG VYSWLPLGLR VLRKIENIVR 70 80 90 100 110 120 EEMNAIGGQE ILLPALLPRA PYETTNRWTE YGDSLFRLKD RRDNDMMLGP THEELFALTV 130 140 150 160 170 180 KGEYSSYKDF PVILYQVQTK YRDEARPRAG ILRGREFVMK DSYSFDTSDD GLKAAYHAHR 190 200 210 220 230 240 DAYQRIFGRL GLDYVIVAAT SGAMGGSASE EFLAESPTGE DTFVRCVESG YAANVEAVIT 250 260 270 280 290 300 PAPPARPIEG LAEAVVHETG DTPTIATLVD WANSAGLGRT VTAADTLKNI LLKVRQPGGD 310 320 330 340 350 360 WELLAVGVPG DREVDDKRLG AALEPAEYEL LGDADFAKYP FLVRGYIGPK ALLANGVRYL 370 380 390 400 410 420 VDPRVVEGTS WITGADEPGK HVVDLVAGRD FTADGTIEAA EVRDGDPSPD GAGQLVSARG 430 440 450 460 470 480 IEIGHIFQLG RKYTDAFTVD VLGENGKPVR LTQGSYGIGV SRLVAVVAEQ QHDELGLRWP 490 500 510 520 530 540 SAVSPFDVHV VIANKDDAAR AGAEELAAEL DRLGHEVLLD DRTASPGVKF KDAELLGVPW 550 560 570 580 IVVIGRGWAD GTIELRNRFT GETQPIAVTD AVASVTQAIG

References

[1]

"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus." Genoscope Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19977 / DSM 44196.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU458896 Genomic DNA. Translation: CAM63217.1.
RefSeq	YP_001703871.1. NC_010397.1.
3D structure databases
ProteinModelPortal	B1MD96.
ModBase	Search...
Protein-protein interaction databases
STRING	B1MD96.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000006558; EBMYCP00000006497; EBMYCG00000006556.
GeneID	5965645.
GenomeReviews	Gene locus MAB_3140c in contig CU458896_GR.
KEGG	mab:MAB_3140c.
PATRIC	17978044. VBIMycAbs55940_3195.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016802.
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Enzyme and pathway databases
BioCyc	MABS561007:MAB_3140C-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_MYCA9

Accession

Primary (citable) accession number: B1MD96

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program