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B1MD72 (TRUB_MYCA9) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase B
EC=5.4.99.25
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name=Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase
Gene names
Name:truB
Ordered Locus Names:MAB_3116c
OrganismMycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]
Taxonomic identifier561007 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01080

Catalytic activity

tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01080

Sequence similarities

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 298298tRNA pseudouridine synthase B HAMAP-Rule MF_01080
PRO_1000149825

Sites

Active site441Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
B1MD72 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: EF93808A0497392D

FASTA29831,362
        10         20         30         40         50         60 
MTDPLSRAGL VIVDKPAGMT SHDVVSRCRR IFSTRKVGHA GTLDPMATGV LVIGIERATK 

        70         80         90        100        110        120 
IMGLLTLTTK SYAATIRFGQ STSTDDAEGE VLQTISASHL TEVDIEAAVA GLRGDIAQVP 

       130        140        150        160        170        180 
SSVSAIKVDG QRAYKLAREG QSVELAARPV RISRFDVLAV RGSGEDLIDV DVEIDCSSGT 

       190        200        210        220        230        240 
YIRALARDAG AALGVGGHLT ALRRTRVGDF GLDRARTLEE LADGATLTLD LDSACRQGFP 

       250        260        270        280        290 
RRDLDAAEVD AVRNGRPLAP AGIEGTYAAV DADDRVIALL ADKGSRTTSV VVLRPANL

« Hide

References

[1]"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus."
Genoscope
Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19977 / DSM 44196.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU458896 Genomic DNA. Translation: CAM63193.1.
RefSeqYP_001703847.1. NC_010397.1.

3D structure databases

ProteinModelPortalB1MD72.
SMRB1MD72. Positions 9-296.
ModBaseSearch...

Protein-protein interaction databases

STRING561007.MAB_3116c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAM63193; CAM63193; MAB_3116c.
GeneID5965621.
KEGGmab:MAB_3116c.
PATRIC17977990. VBIMycAbs55940_3168.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0130.
HOGENOMHOG000231225.
KOK03177.
OMAKKSFRNE.
ProtClustDBPRK03287.

Enzyme and pathway databases

BioCycMABS561007:GJTG-3131-MONOMER.

Family and domain databases

HAMAPMF_01080. TruB_bact.
InterProIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR015947. PUA-like_domain.
IPR015225. tRNA_psdUridine_synth_fam2_C.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERPTHR13767. PTHR13767. 1 hit.
PfamPF09142. TruB_C. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMSSF55120. PsdUridine_synth_cat_dom. 1 hit.
SSF88697. PUA-like. 1 hit.
TIGRFAMsTIGR00431. TruB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRUB_MYCA9
AccessionPrimary (citable) accession number: B1MD72
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 29, 2008
Last modified: May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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