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B1MD00

- DAPF_MYCA9

UniProt

B1MD00 - DAPF_MYCA9

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Mycobacterium abscessus (strain ATCC 19977 / DSM 44196)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111SubstrateUniRule annotation
    Binding sitei44 – 441SubstrateUniRule annotation
    Binding sitei78 – 781SubstrateUniRule annotation
    Active sitei87 – 871Proton donor/acceptorUniRule annotation
    Binding sitei163 – 1631SubstrateUniRule annotation
    Sitei165 – 1651Important for catalytic activityUniRule annotation
    Binding sitei199 – 1991SubstrateUniRule annotation
    Sitei220 – 2201Important for catalytic activityUniRule annotation
    Active sitei229 – 2291Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciMABS36809-WGS:GSQO-3059-MONOMER.
    MABS561007:GJTG-3059-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:MAB_3043c
    OrganismiMycobacterium abscessus (strain ATCC 19977 / DSM 44196)
    Taxonomic identifieri561007 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium abscessus
    ProteomesiUP000007137: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 295295Diaminopimelate epimerasePRO_1000099249Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi87 ↔ 229

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi561007.MAB_3043c.

    Structurei

    3D structure databases

    ProteinModelPortaliB1MD00.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni87 – 893Substrate bindingUniRule annotation
    Regioni220 – 2212Substrate bindingUniRule annotation
    Regioni230 – 2312Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220467.
    KOiK01778.
    OMAiITIEMGT.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1MD00-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFTKGHGTQ NDFVVLPDVH IKRDLSVAAV QALCDRQRGL GADGVLRVTT    50
    VGAALEGGVL AEKPAGVSSE DWFMDYRNAD GSIAEMCGNG VRVFAHYLRS 100
    VGLEHRDEFV VASLAGPRPV RINSWSQLTA DVTVDMGPVK EFGAGEAIVG 150
    GRRFSGLAID VGNPHLACVD AVLTTEQLRI LDVGAPVTFD EQLFPDGVNV 200
    EVLTAPSAGP ANTVHMRVHE RGVGETRSCG TGTVAAAYAA LRHIGQRTGE 250
    VVVNIPGGQV RVTVTGESSF LRGPSMLLAD GEISDEWWGG IGACG 295
    Length:295
    Mass (Da):31,029
    Last modified:April 29, 2008 - v1
    Checksum:iF35741A749DCDDFA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU458896 Genomic DNA. Translation: CAM63121.1.
    RefSeqiYP_001703775.1. NC_010397.1.

    Genome annotation databases

    EnsemblBacteriaiCAM63121; CAM63121; MAB_3043c.
    GeneIDi5965550.
    KEGGimab:MAB_3043c.
    PATRICi17977842. VBIMycAbs55940_3094.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU458896 Genomic DNA. Translation: CAM63121.1 .
    RefSeqi YP_001703775.1. NC_010397.1.

    3D structure databases

    ProteinModelPortali B1MD00.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 561007.MAB_3043c.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAM63121 ; CAM63121 ; MAB_3043c .
    GeneIDi 5965550.
    KEGGi mab:MAB_3043c.
    PATRICi 17977842. VBIMycAbs55940_3094.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220467.
    KOi K01778.
    OMAi ITIEMGT.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci MABS36809-WGS:GSQO-3059-MONOMER.
    MABS561007:GJTG-3059-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus."
      Genoscope
      Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L.
      Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 19977 / DSM 44196.

    Entry informationi

    Entry nameiDAPF_MYCA9
    AccessioniPrimary (citable) accession number: B1MD00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3