B1MCU7 (DXS_MYCA9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	MAB_2990c

Organism

Mycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]

Taxonomic identifier

561007 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 641	641	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000115751

Sequences

	Sequence		Length	Mass (Da)
	B1MCU7 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 9460719EBD753238	FASTA	641	68,267
	10 20 30 40 50 60 MLDDIRGPGD LQGLSKRELD LLADEIREFL IHKVAATGGH LGPNLGVVEL SLALHRVFES 70 80 90 100 110 120 PYDPIIFDTG HQAYVHKILT GRAGDFDTLR QKDGLSGYPS RAESEHDWVE SSHASTALSY 130 140 150 160 170 180 ADGLAKAFEL TGQRRRHVVA VVGDGAMTGG MCWEALNNIA AAEHRRIIIV VNDNGRSYAP 190 200 210 220 230 240 TIGGLASHLA GLRLTPSYEK VLDESKKLLR GMPVVGPLAY ALMHSLKVGV KDAVSPQVMF 250 260 270 280 290 300 TDLGLKYVGP VDGHDEHAVE DALRRARGYG GPVIVHVITR KGMGYAPAED DEAEQMHSTG 310 320 330 340 350 360 IIDPKTGRAI KASAPGWTSV FSDALIDVAS EHRNIVAITA AMPGPTGLSK FGERFPDRLF 370 380 390 400 410 420 DVGIAEQHAV TSAAGLAMGG LHPVVAIYST FLNRAFDQVM MDVALHRLPV TFVLDRAGIT 430 440 450 460 470 480 GSDGASHNGM WDLSILNVVP GMRVAAPRDA SRLREELTEA LAVDDGPTAL RFPKGEVGED 490 500 510 520 530 540 IPAIERRDGV DILARPASGL HADVLLVAVG SFASTALATA DRLGKQGIGV TVVDPRWVLP 550 560 570 580 590 600 VPPHVVELAG QHRLVVTLED SGVHGGAGSA VTAAMQDADV DIPSRDIGVP QQFLEHASRG 610 620 630 640 QVLEELGLTD QHIARKITGW VAAMSREIGD STVNPAAQQV D

References

[1]

"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus." Genoscope Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19977 / DSM 44196.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU458896 Genomic DNA. Translation: CAM63068.1.
RefSeq	YP_001703722.1. NC_010397.1.
3D structure databases
ProteinModelPortal	B1MCU7.
ModBase	Search...
Protein-protein interaction databases
STRING	B1MCU7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000004992; EBMYCP00000004931; EBMYCG00000004990.
GeneID	5965497.
GenomeReviews	Gene locus MAB_2990c in contig CU458896_GR.
KEGG	mab:MAB_2990c.
PATRIC	17977734. VBIMycAbs55940_3040.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016269.
HOGENOM	HBG571647.
OMA	QYDISYM.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	MABS561007:MAB_2990C-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_MYCA9

Accession

Primary (citable) accession number: B1MCU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program