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Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Mycobacterium abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei80NucleophileUniRule annotation1
Binding sitei109L-glutamineUniRule annotation1
Active sitei173Charge relay systemUniRule annotation1
Active sitei175Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00245

Protein family/group databases

MEROPSiC26.A32

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxTUniRule annotation (EC:4.3.3.6UniRule annotation)
Alternative name(s):
Pdx2UniRule annotation
Pyridoxal 5'-phosphate synthase glutaminase subunitUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:pdxTUniRule annotation
Ordered Locus Names:MAB_2890c
OrganismiMycobacterium abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543)
Taxonomic identifieri561007 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteroides abscessus
Proteomesi
  • UP000007137 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002157191 – 193Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST193

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.UniRule annotation

Protein-protein interaction databases

STRINGi561007.MAB_2890c

Structurei

3D structure databases

ProteinModelPortaliB1MCJ8
SMRiB1MCJ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 50L-glutamine bindingUniRule annotation3
Regioni137 – 138L-glutamine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4108UHX Bacteria
COG0311 LUCA
HOGENOMiHOG000039949
OMAiVFIRAPI

Family and domain databases

CDDicd01749 GATase1_PB, 1 hit
Gene3Di3.40.50.880, 1 hit
HAMAPiMF_01615 PdxT, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR002161 PdxT/SNO
IPR021196 PdxT/SNO_CS
PANTHERiPTHR31559 PTHR31559, 1 hit
PfamiView protein in Pfam
PF01174 SNO, 1 hit
PIRSFiPIRSF005639 Glut_amidoT_SNO, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR03800 PLP_synth_Pdx2, 1 hit
PROSITEiView protein in PROSITE
PS01236 PDXT_SNO_1, 1 hit
PS51130 PDXT_SNO_2, 1 hit

Sequencei

Sequence statusi: Complete.

B1MCJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPLVGVLAL QGDVREHVAA LKDSGAEALG VRRPEELGKV DGLVIPGGES
60 70 80 90 100
TTMSNLLRVF ELLDPLTERL RAGLPVYGSC AGMILLASEI LDTRPDAVAL
110 120 130 140 150
GAIDMTVRRN AFGRQVDSFE GDLDFTGVDG AMHAVFIRAP WVERVGDDVE
160 170 180 190
VLASAQGHPV AVRQGSALAT AFHPEVTGDR RVHQLFVDMV RAG
Length:193
Mass (Da):20,536
Last modified:April 29, 2008 - v1
Checksum:i6FA0F1163C37D2F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU458896 Genomic DNA Translation: CAM62969.1

Genome annotation databases

EnsemblBacteriaiCAM62969; CAM62969; MAB_2890c

Similar proteinsi

Entry informationi

Entry nameiPDXT_MYCA9
AccessioniPrimary (citable) accession number: B1MCJ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: April 29, 2008
Last modified: June 20, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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