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B1MBZ5 (BIOD_MYCA9) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3
Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase
Gene names
Name:bioD
Ordered Locus Names:MAB_2686c
OrganismMycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]
Taxonomic identifier561007 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length225 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336

Catalytic activity

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336

Cofactor

Magnesium By similarity. HAMAP MF_00336

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336

Subcellular location

Cytoplasm By similarity HAMAP MF_00336.

Sequence similarities

Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

dethiobiotin synthase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 225225ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336
PRO_1000119876

Regions

Nucleotide binding105 – 1084ATP By similarity
Nucleotide binding196 – 1983ATP By similarity

Sites

Metal binding161Magnesium 2 By similarity
Metal binding461Magnesium 2 By similarity
Metal binding1051Magnesium 2 By similarity
Binding site411Substrate By similarity
Binding site461ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1MBZ5 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 0611F265B1BD2740

FASTA22523,095
        10         20         30         40         50         60 
MTILLVTGTS TGVGKTVATA ALAAAAVRQG IDVTVCKPVQ TGDDHDAGEV ARLSGVTRVQ 

        70         80         90        100        110        120 
TLVRYPEPLA PVASASRAGL ELLDHTQMAT AIVALDRPGA LTLVEGAGGL LVELAADGKT 

       130        140        150        160        170        180 
LRDLAMVLDA PVLVVTTADL GTLNHTALTL EALAVQSVPC AGLVVGSFPA EPDLAQRLNR 

       190        200        210        220 
ENLANQFGTP VRAVIPEGAA RLMPPVFAEL SIELFEPQWV AGLVA

« Hide

References

[1]"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus."
Genoscope
Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19977 / DSM 44196.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU458896 Genomic DNA. Translation: CAM62766.1.
RefSeqYP_001703420.1. NC_010397.1.

3D structure databases

ProteinModelPortalB1MBZ5.
ModBaseSearch...

Protein-protein interaction databases

STRINGB1MBZ5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000008235; EBMYCP00000008174; EBMYCG00000008233.
GeneID5965195.
GenomeReviewsGene locus MAB_2686c in contig CU458896_GR.
KEGGmab:MAB_2686c.
PATRIC17977106. VBIMycAbs55940_2730.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000017652.
HOGENOMHBG650065.
OMAAEAVGWC.
ProtClustDBPRK00090.

Enzyme and pathway databases

BioCycMABS561007:MAB_2686C-MONOMER.

Family and domain databases

HAMAPMF_00336. BioD.
[Tree]
InterProIPR002586. CbiA_P_synth.
IPR004472. DTB_synth_BioD.
[Graphical view]
KOK01935.
PfamPF01656. CbiA. 1 hit.
[Graphical view]
PIRSFPIRSF006755. DTB_synth. 1 hit.
TIGRFAMsTIGR00347. BioD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOD_MYCA9
AccessionPrimary (citable) accession number: B1MBZ5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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