Reviewed, UniProtKB/Swiss-Prot B1MB69 (MASZ_MYCA9)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	MAB_2409c

Organism

Mycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]

Taxonomic identifier

561007 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Malate synthase G HAMAP MF_00641		PRO_1000130892
Sites
	Active site	346	1	Proton acceptor By similarity
	Active site	641	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1MB69-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 29D2078E5D4D336E	FASTA	735	80,389
	10 20 30 40 50 60 MSDRVDVGNL RVARVLYDFI TNEALPGTGV DADAFWAGVD KVVTDLAPRN RELLERRDDL 70 80 90 100 110 120 QTQVDRWHRQ RAIEPLDGAA YQDFLTEIGY LVPEPEDFTI TTANVDDEIT TTAGPQLVVP 130 140 150 160 170 180 ILNARFALNA ANARWGSLYD ALYGTDVIPE ADGAEKASAD TGGYNKVRGD KVIAYAREVL 190 200 210 220 230 240 DGAAPLASGS WKDAVGLKID DGQLLVELGD EFSTGLQNPD QFVGYTGKLG KPQWSVLLIN 250 260 270 280 290 300 HGLHIEVLID PDSPIGSTDK AGIKDVVLES AITTIMDFED SVAAVDAEDK VLGYRNWLGL 310 320 330 340 350 360 NKGDLSEEVS KGGKTFTRVL NEDRVFTTPD GKGELTLPGR SLLFVRNVGH LMTNDAIVDA 370 380 390 400 410 420 AGNEMPEGIQ DALFTSLIAI HGLRSGKKNG PLKNSRTGSI YIVKPKMHGP EEVAFTAELF 430 440 450 460 470 480 GRVEEVLGLP HATLKVGIMD EERRTTVNLK AAIKAASDRV VFINTGFLDR TGDEIHTSME 490 500 510 520 530 540 AGPMCRKAVM KSQPWILAYE DHNVDTGLGA GLAGKAQIGK GMWAMPDLMA DMVAQKIAQP 550 560 570 580 590 600 RAGATTAWVP SPTAATLHAL HYHQVDVKAV DQELAGKHRA KLESLLTIPL AESRHDWTAE 610 620 630 640 650 660 DIREEIDNNC QSILGYVVRW IDQGVGCSKV PDIHDVALME DRATLRISSQ LLANWLRHDV 670 680 690 700 710 720 ITEDEVVEGL KRMAPVVDRQ NAGDKEYRPM APDFDSNIAF QAAKELILEG KDQPNGYTEP 730 ILHRRRREYK AAAAK

References

[1]

"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus." Genoscope Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU458896 Genomic DNA. Translation: CAM62490.1.
RefSeq	YP_001703144.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5964919.
GenomeReviews	Gene locus MAB_2409c in contig CU458896_GR.
KEGG	mab:MAB_2409c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PKMHGPD.
Family and domain databases
HAMAP	MF_00641. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_MYCA9

Accession

Primary (citable) accession number: B1MB69

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)