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B1MB69

- MASZ_MYCA9

UniProt

B1MB69 - MASZ_MYCA9

Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium abscessus (strain ATCC 19977 / DSM 44196)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei281 – 2811Acetyl-CoAUniRule annotation
    Binding sitei318 – 3181Acetyl-CoAUniRule annotation
    Active sitei346 – 3461Proton acceptorUniRule annotation
    Binding sitei346 – 3461GlyoxylateUniRule annotation
    Metal bindingi441 – 4411MagnesiumUniRule annotation
    Binding sitei441 – 4411GlyoxylateUniRule annotation
    Metal bindingi469 – 4691MagnesiumUniRule annotation
    Binding sitei550 – 5501Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei641 – 6411Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMABS36809-WGS:GSQO-2423-MONOMER.
    MABS561007:GJTG-2423-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:MAB_2409c
    OrganismiMycobacterium abscessus (strain ATCC 19977 / DSM 44196)
    Taxonomic identifieri561007 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium abscessus
    ProteomesiUP000007137: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 735735Malate synthase GPRO_1000130892Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei627 – 6271Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi561007.MAB_2409c.

    Structurei

    3D structure databases

    ProteinModelPortaliB1MB69.
    SMRiB1MB69. Positions 5-735.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni466 – 4694Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B1MB69-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDRVDVGNL RVARVLYDFI TNEALPGTGV DADAFWAGVD KVVTDLAPRN    50
    RELLERRDDL QTQVDRWHRQ RAIEPLDGAA YQDFLTEIGY LVPEPEDFTI 100
    TTANVDDEIT TTAGPQLVVP ILNARFALNA ANARWGSLYD ALYGTDVIPE 150
    ADGAEKASAD TGGYNKVRGD KVIAYAREVL DGAAPLASGS WKDAVGLKID 200
    DGQLLVELGD EFSTGLQNPD QFVGYTGKLG KPQWSVLLIN HGLHIEVLID 250
    PDSPIGSTDK AGIKDVVLES AITTIMDFED SVAAVDAEDK VLGYRNWLGL 300
    NKGDLSEEVS KGGKTFTRVL NEDRVFTTPD GKGELTLPGR SLLFVRNVGH 350
    LMTNDAIVDA AGNEMPEGIQ DALFTSLIAI HGLRSGKKNG PLKNSRTGSI 400
    YIVKPKMHGP EEVAFTAELF GRVEEVLGLP HATLKVGIMD EERRTTVNLK 450
    AAIKAASDRV VFINTGFLDR TGDEIHTSME AGPMCRKAVM KSQPWILAYE 500
    DHNVDTGLGA GLAGKAQIGK GMWAMPDLMA DMVAQKIAQP RAGATTAWVP 550
    SPTAATLHAL HYHQVDVKAV DQELAGKHRA KLESLLTIPL AESRHDWTAE 600
    DIREEIDNNC QSILGYVVRW IDQGVGCSKV PDIHDVALME DRATLRISSQ 650
    LLANWLRHDV ITEDEVVEGL KRMAPVVDRQ NAGDKEYRPM APDFDSNIAF 700
    QAAKELILEG KDQPNGYTEP ILHRRRREYK AAAAK 735
    Length:735
    Mass (Da):80,389
    Last modified:April 29, 2008 - v1
    Checksum:i29D2078E5D4D336E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU458896 Genomic DNA. Translation: CAM62490.1.
    RefSeqiYP_001703144.1. NC_010397.1.

    Genome annotation databases

    EnsemblBacteriaiCAM62490; CAM62490; MAB_2409c.
    GeneIDi5964919.
    KEGGimab:MAB_2409c.
    PATRICi17976552. VBIMycAbs55940_2453.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU458896 Genomic DNA. Translation: CAM62490.1 .
    RefSeqi YP_001703144.1. NC_010397.1.

    3D structure databases

    ProteinModelPortali B1MB69.
    SMRi B1MB69. Positions 5-735.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 561007.MAB_2409c.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAM62490 ; CAM62490 ; MAB_2409c .
    GeneIDi 5964919.
    KEGGi mab:MAB_2409c.
    PATRICi 17976552. VBIMycAbs55940_2453.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci MABS36809-WGS:GSQO-2423-MONOMER.
    MABS561007:GJTG-2423-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus."
      Genoscope
      Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L.
      Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 19977 / DSM 44196.

    Entry informationi

    Entry nameiMASZ_MYCA9
    AccessioniPrimary (citable) accession number: B1MB69
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3