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Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei281Acetyl-CoAUniRule annotation1
Binding sitei318Acetyl-CoAUniRule annotation1
Active sitei346Proton acceptorUniRule annotation1
Binding sitei346GlyoxylateUniRule annotation1
Metal bindingi441MagnesiumUniRule annotation1
Binding sitei441GlyoxylateUniRule annotation1
Metal bindingi469MagnesiumUniRule annotation1
Binding sitei550Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei641Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMABS36809:G12XF-2423-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:MAB_2409c
OrganismiMycobacterium abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543)
Taxonomic identifieri561007 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium abscessus
Proteomesi
  • UP000007137 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001308921 – 735Malate synthase GAdd BLAST735

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei627Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi561007.MAB_2409c.

Structurei

3D structure databases

ProteinModelPortaliB1MB69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni466 – 469Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
OMAiTEPVLHA.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

B1MB69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDRVDVGNL RVARVLYDFI TNEALPGTGV DADAFWAGVD KVVTDLAPRN
60 70 80 90 100
RELLERRDDL QTQVDRWHRQ RAIEPLDGAA YQDFLTEIGY LVPEPEDFTI
110 120 130 140 150
TTANVDDEIT TTAGPQLVVP ILNARFALNA ANARWGSLYD ALYGTDVIPE
160 170 180 190 200
ADGAEKASAD TGGYNKVRGD KVIAYAREVL DGAAPLASGS WKDAVGLKID
210 220 230 240 250
DGQLLVELGD EFSTGLQNPD QFVGYTGKLG KPQWSVLLIN HGLHIEVLID
260 270 280 290 300
PDSPIGSTDK AGIKDVVLES AITTIMDFED SVAAVDAEDK VLGYRNWLGL
310 320 330 340 350
NKGDLSEEVS KGGKTFTRVL NEDRVFTTPD GKGELTLPGR SLLFVRNVGH
360 370 380 390 400
LMTNDAIVDA AGNEMPEGIQ DALFTSLIAI HGLRSGKKNG PLKNSRTGSI
410 420 430 440 450
YIVKPKMHGP EEVAFTAELF GRVEEVLGLP HATLKVGIMD EERRTTVNLK
460 470 480 490 500
AAIKAASDRV VFINTGFLDR TGDEIHTSME AGPMCRKAVM KSQPWILAYE
510 520 530 540 550
DHNVDTGLGA GLAGKAQIGK GMWAMPDLMA DMVAQKIAQP RAGATTAWVP
560 570 580 590 600
SPTAATLHAL HYHQVDVKAV DQELAGKHRA KLESLLTIPL AESRHDWTAE
610 620 630 640 650
DIREEIDNNC QSILGYVVRW IDQGVGCSKV PDIHDVALME DRATLRISSQ
660 670 680 690 700
LLANWLRHDV ITEDEVVEGL KRMAPVVDRQ NAGDKEYRPM APDFDSNIAF
710 720 730
QAAKELILEG KDQPNGYTEP ILHRRRREYK AAAAK
Length:735
Mass (Da):80,389
Last modified:April 29, 2008 - v1
Checksum:i29D2078E5D4D336E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU458896 Genomic DNA. Translation: CAM62490.1.

Genome annotation databases

EnsemblBacteriaiCAM62490; CAM62490; MAB_2409c.
PATRICi17976552. VBIMycAbs55940_2453.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU458896 Genomic DNA. Translation: CAM62490.1.

3D structure databases

ProteinModelPortaliB1MB69.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi561007.MAB_2409c.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM62490; CAM62490; MAB_2409c.
PATRICi17976552. VBIMycAbs55940_2453.

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
OMAiTEPVLHA.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciMABS36809:G12XF-2423-MONOMER.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMASZ_MYCA9
AccessioniPrimary (citable) accession number: B1MB69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: November 30, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.