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Protein

Catalase-peroxidase

Gene

katG

Organism
Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei105Transition state stabilizerUniRule annotation1
Active sitei109Proton acceptorUniRule annotation1
Metal bindingi288Iron (heme axial ligand)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Ordered Locus Names:Mrad2831_4830
OrganismiMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Taxonomic identifieri426355 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000006589 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003548331 – 772Catalase-peroxidaseAdd BLAST772

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki108 ↔ 247Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-273)UniRule annotation
Cross-linki247 ↔ 273Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-108)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Proteomic databases

PRIDEiB1M869.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi426355.Mrad2831_4830.

Structurei

3D structure databases

ProteinModelPortaliB1M869.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiYGATTMG.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1M869-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETIDKTDT TTFHMEGKCP FGGDRIGGAL GNRPTLENWY PHRLRVEVLH
60 70 80 90 100
QNGLAADPLG PDFDYAAEFA KIDFEALKRD IKQFLTSSVD WWPSDYGNYG
110 120 130 140 150
PQMIRMAWHS AGTYRIADGR GGAGTGLQRF APISSWWDNG NTDKSRRLLQ
160 170 180 190 200
PIKHKYGNAL SWADLMVLTG NCALEIMGLP TYGFAAGRLD AWEADNATYW
210 220 230 240 250
GPEVVEMGSV SSFDDMVNRD KRWRGKNGDA DYDLENPLAA SHQALIYVNP
260 270 280 290 300
EGPYASGDPL ASARDIRITF TRMAMNDEET VALIAGGHAF GKSHGMTPAK
310 320 330 340 350
EIGPPPEMAP MEAMGLGWHN PKGSGAGKDT MTNGIEGSWT PDPTKWDNAY
360 370 380 390 400
LENLFRFEWE QTRSPAGALQ WTPKDPDAPK TPDAHVAGQM HPLMMMTSDI
410 420 430 440 450
ALKVDPDYRK VCEKFLNDFD AFTQAFSKAW YKLTHRDMGP KHRYLGPEAV
460 470 480 490 500
IEDGLLWQDP LPERDYALVG EAEIAALKQA IAATGLSVSD LAFTAFSAAS
510 520 530 540 550
TYRDSDKRGG ANGGRLALAP QKDWAVNRRA APVVEALRGV MATFNDGRSD
560 570 580 590 600
GKKISLADLI VLGGCVAVER AARDAGVETP VPFTPGRVDT TQALTDLEMF
610 620 630 640 650
EWLKPVVDGF RNYVDDGFGQ MTRSVSPEEM FLDKANLLTL TAPEWTVLTG
660 670 680 690 700
GLRALNANHD GSNRGVLTDR VGVLTTDFFR NLTDVDLVWE KADADGMTFA
710 720 730 740 750
LKDRASGQTK FEATRSDLVF GSNAQLRSIA DAYAGSDGQR RFVADFVKAW
760 770
DKVMMLDRFD VKGHRRYGPM AA
Length:772
Mass (Da):84,977
Last modified:April 29, 2008 - v1
Checksum:i309769DFC5486317
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA. Translation: ACB26790.1.
RefSeqiWP_012321741.1. NC_010505.1.

Genome annotation databases

EnsemblBacteriaiACB26790; ACB26790; Mrad2831_4830.
GeneIDi6140898.
KEGGimrd:Mrad2831_4830.
PATRICi22578076. VBIMetRad70578_4898.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA. Translation: ACB26790.1.
RefSeqiWP_012321741.1. NC_010505.1.

3D structure databases

ProteinModelPortaliB1M869.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi426355.Mrad2831_4830.

Proteomic databases

PRIDEiB1M869.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB26790; ACB26790; Mrad2831_4830.
GeneIDi6140898.
KEGGimrd:Mrad2831_4830.
PATRICi22578076. VBIMetRad70578_4898.

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiYGATTMG.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKATG_METRJ
AccessioniPrimary (citable) accession number: B1M869
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.