B1M860 (DADA_METRJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-amino acid dehydrogenase small subunit
EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	Mrad2831_4821

Organism

Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP]

Taxonomic identifier

426355 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity. HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity. HAMAP MF_01202
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the DadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000138657
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	B1M860 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: BBE4834C3DE85080	FASTA	419	45,004
	10 20 30 40 50 60 MHVLILGGGV VGVTSAYYLA RAGHQVTVLE RQPGAGLETS FANAGQVSPG YSAPWAAPGI 70 80 90 100 110 120 PVKALRWLMM RHRPLVLWPR LEPRLYAWLT RMLANCTEEA YRRNKGRMVR LAEYSRDALR 130 140 150 160 170 180 DLRTETGIAY DHREKGTLQL FRTRKQLDHV GDDTRVLDAY GVPYTVLDPA GCIAAEPALA 190 200 210 220 230 240 AVRDVFVGGL RLPGDETGDA HLFTQRLAAL CESLGVTFRY GTAIARLHHA GDRVTAVETA 250 260 270 280 290 300 DGALLRADAY VAALGSYTPA LLRPLGIALP VYPVKGYSLT LPITDAEAAP VSTVMDETYK 310 320 330 340 350 360 VAITRLGDRI RVGGTAELAG FSNALRGPRR ETLARSVQDL FPAGGDLDKA SFWTGLRPMT 370 380 390 400 410 PDGTPIVGGT RVGNLFTNTG HGTLGWTMAC GSGRLLADLV SGRAPEIASD DLALGRYAA

References

[1]

"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C.J., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001001 Genomic DNA. Translation: ACB26781.1.
RefSeq	YP_001757464.1. NC_010505.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B1M860.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6140889.
GenomeReviews	Gene locus Mrad2831_4821 in contig CP001001_GR.
KEGG	mrd:Mrad2831_4821.
PATRIC	22578058. VBIMetRad70578_4889.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG729204.
OMA	VDRQPAV.
ProtClustDB	CLSK2328131.
Family and domain databases
HAMAP	MF_01202. DadA. [Tree]
InterPro	IPR023080. D-aa_DH_ssu_DadA. IPR006076. FAD-dep_OxRdtase. [Graphical view]
KO	K00285.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_METRJ

Accession

Primary (citable) accession number: B1M860

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program