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Protein

Orotidine 5'-phosphate decarboxylase

Gene

pyrF

Organism
Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16SubstrateUniRule annotation1
Binding sitei38SubstrateUniRule annotation1
Active sitei67Proton donorUniRule annotation1
Binding sitei119SubstrateUniRule annotation1
Binding sitei180SubstrateUniRule annotation1
Binding sitei189SubstrateUniRule annotation1
Binding sitei209Substrate; via amide nitrogenUniRule annotation1
Binding sitei210SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processPyrimidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00070; UER00120

Names & Taxonomyi

Protein namesi
Recommended name:
Orotidine 5'-phosphate decarboxylaseUniRule annotation (EC:4.1.1.23UniRule annotation)
Alternative name(s):
OMP decarboxylaseUniRule annotation
Short name:
OMPDCaseUniRule annotation
Short name:
OMPdecaseUniRule annotation
Gene namesi
Name:pyrFUniRule annotation
Ordered Locus Names:Mrad2831_1753
OrganismiMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1)
Taxonomic identifieri426355 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001385421 – 230Orotidine 5'-phosphate decarboxylaseAdd BLAST230

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi426355.Mrad2831_1753

Structurei

3D structure databases

ProteinModelPortaliB1M7M3
SMRiB1M7M3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 74Substrate bindingUniRule annotation10

Sequence similaritiesi

Belongs to the OMP decarboxylase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106EG9 Bacteria
COG0284 LUCA
HOGENOMiHOG000226070
KOiK01591
OMAiNFKIFLD
OrthoDBiPOG091H0256

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01200_B OMPdecase_type1_B, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR014732 OMPdecase
IPR018089 OMPdecase_AS
IPR001754 OMPdeCOase_dom
IPR011060 RibuloseP-bd_barrel
PANTHERiPTHR32119:SF2 PTHR32119:SF2, 1 hit
PfamiView protein in Pfam
PF00215 OMPdecase, 1 hit
SMARTiView protein in SMART
SM00934 OMPdecase, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR01740 pyrF, 1 hit
PROSITEiView protein in PROSITE
PS00156 OMPDECASE, 1 hit

Sequencei

Sequence statusi: Complete.

B1M7M3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEIADPRDR LIVALDLPSV QAAEALIDRI GDAATFYKIG YQLGYAGGLA
60 70 80 90 100
LAERLAARGL KVFLDLKLHD IGNTVEEGVR SASGSGATFL TVHAYPQTMR
110 120 130 140 150
AAVRGRGAGL RILAVTVLTS YDDADAAEAG YGLPVAELVA KRAAQAAEIG
160 170 180 190 200
IDGLVCSAAE AGSVRRIVGP DRLIVTPGIR PAGAEAGDQK RVMTPGDARR
210 220 230
AGIDHVVVGR PITGAADPRA VAQAIVADLA
Length:230
Mass (Da):23,701
Last modified:April 29, 2008 - v1
Checksum:i21CC0609A8A2C687
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA Translation: ACB23748.1
RefSeqiWP_012318735.1, NC_010505.1

Genome annotation databases

EnsemblBacteriaiACB23748; ACB23748; Mrad2831_1753
GeneIDi6137782
KEGGimrd:Mrad2831_1753
PATRICifig|426355.14.peg.1801

Similar proteinsi

Entry informationi

Entry nameiPYRF_METRJ
AccessioniPrimary (citable) accession number: B1M7M3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: May 23, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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