B1M6N7 (B1M6N7_METRJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Peptide deformylase 2 HAMAP MF_00163
Short name=PDF 2 HAMAP MF_00163
EC=3.5.1.88 HAMAP MF_00163

Alternative name(s):
Polypeptide deformylase 2 HAMAP MF_00163

Gene names

Name:	def2 HAMAP MF_00163
Ordered Locus Names:	Mrad2831_3146

Organism

Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP]

Taxonomic identifier

426355 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163 SAAS SAAS000181
Cofactor	Binds 1 Fe2+ ion By similarity. HAMAP MF_00163
Sequence similarities	Belongs to the polypeptide deformylase family. HAMAP MF_00163 RuleBase RU003335

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00163 SAAS SAAS000181
Ligand	Iron HAMAP MF_00163 Metal-binding HAMAP MF_00163 SAAS SAAS000181
Molecular function	Hydrolase SAAS SAAS000181 HAMAP MF_00163 EMBL ACB25128.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	137	1	By similarity HAMAP MF_00163
	Metal binding	94	1	Iron By similarity HAMAP MF_00163
	Metal binding	136	1	Iron By similarity HAMAP MF_00163
	Metal binding	140	1	Iron By similarity HAMAP MF_00163

Sequences

	Sequence		Length	Mass (Da)
	B1M6N7 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 3F8AA08F9AFF7C42	FASTA	171	19,257
	10 20 30 40 50 60 MTIRPLVILP DPVLRLGSEP VGPITAEIRT LVADMFETMY DAPGVGLAAI QIGVPKRVVT 70 80 90 100 110 120 IDTSKEEGVR DARVFINPEI VWSSEEKRVY DEGCLSIPDY YAEVERPDRV RVKFRDLDGT 130 140 150 160 170 EREIEADGLL STCIQHEIDH LNGVLFIDHL SKLKRDRVIK KFTKAAKRDA A

References

[1]

"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C.J., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001001 Genomic DNA. Translation: ACB25128.1.
RefSeq	YP_001755811.1. NC_010505.1.
3D structure databases
ProteinModelPortal	B1M6N7.
SMR	B1M6N7. Positions 2-170.
ModBase	Search...
Protein-protein interaction databases
STRING	B1M6N7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6139193.
GenomeReviews	Gene locus Mrad2831_3146 in contig CP001001_GR.
KEGG	mrd:Mrad2831_3146.
PATRIC	22574670. VBIMetRad70578_3216.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG665227.
OMA	RITKVDE.
ProtClustDB	CLSK2330789.
Family and domain databases
HAMAP	MF_00163. Pep_deformylase. [Tree]
InterPro	IPR000181. Fmet_deformylase. IPR023635. Peptide_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KO	K01462.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
SUPFAM	SSF56420. Fmet_deformylase. 1 hit.
TIGRFAMs	TIGR00079. Pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B1M6N7_METRJ

Accession

Primary (citable) accession number: B1M6N7

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)