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Protein

Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB

Gene

rutB

Organism
Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2.UniRule annotation

Catalytic activityi

(Z)-3-ureidoacrylate peracid + H2O = (Z)-3-peroxyaminoacrylate + CO2 + NH3.UniRule annotation
(Z)-3-ureidoacrylate + H2O = (Z)-3-aminoacrylate + CO2 + NH3.UniRule annotation
(Z)-2-methyl-ureidoacrylate peracid + H2O = (Z)-2-methyl-peroxyaminoacrylate + CO2 + NH3.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei189NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBUniRule annotation (EC:3.5.1.110UniRule annotation)
Alternative name(s):
Ureidoacrylate amidohydrolaseUniRule annotation
Gene namesi
Name:rutBUniRule annotation
Ordered Locus Names:Mrad2831_1583
OrganismiMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Taxonomic identifieri426355 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000006589 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004026961 – 258Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBAdd BLAST258

Interactioni

Protein-protein interaction databases

STRINGi426355.Mrad2831_1583.

Structurei

3D structure databases

ProteinModelPortaliB1M6B8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the isochorismatase family. RutB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106XMA. Bacteria.
COG1335. LUCA.
HOGENOMiHOG000078669.
KOiK09020.
OMAiQRDITHI.
OrthoDBiPOG091H0ARF.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB. 1 hit.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.

Sequencei

Sequence statusi: Complete.

B1M6B8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRPTTYPMD QPAGFRDAQG RHGGVVLPAR PEPIALDPAT TFLIVVDVQN
60 70 80 90 100
AYASPGGYLD RAGFDVSGTG PVIARIARAV AAARAAGIPV LWFQNGWDPA
110 120 130 140 150
YVEAGGPGSP NWHKSNALKT MRRNPEANTQ LLAKGSWDYA LVDALTPEPG
160 170 180 190 200
DIVMGKPRYS GFYNTPLDST LRARGVTTLV FTGIATNVCV ESTLRDGFHR
210 220 230 240 250
EYFGIVLADA THQAGPESLH RAALANIETF FGWVSDVAAF ESALGVPSRA

PAGADLAG
Length:258
Mass (Da):27,350
Last modified:April 29, 2008 - v1
Checksum:i2E53F435935B8B3A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA. Translation: ACB23578.1.

Genome annotation databases

EnsemblBacteriaiACB23578; ACB23578; Mrad2831_1583.
KEGGimrd:Mrad2831_1583.
PATRICi22571444. VBIMetRad70578_1621.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA. Translation: ACB23578.1.

3D structure databases

ProteinModelPortaliB1M6B8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi426355.Mrad2831_1583.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB23578; ACB23578; Mrad2831_1583.
KEGGimrd:Mrad2831_1583.
PATRICi22571444. VBIMetRad70578_1621.

Phylogenomic databases

eggNOGiENOG4106XMA. Bacteria.
COG1335. LUCA.
HOGENOMiHOG000078669.
KOiK09020.
OMAiQRDITHI.
OrthoDBiPOG091H0ARF.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB. 1 hit.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUTB_METRJ
AccessioniPrimary (citable) accession number: B1M6B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.