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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 14FADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandFAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:Mrad2831_1573
OrganismiMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1)
Taxonomic identifieri426355 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000006589 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003463591 – 475Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOAdd BLAST475

Proteomic databases

PRIDEiB1M5H7

Interactioni

Protein-protein interaction databases

STRINGi426355.Mrad2831_1573

Structurei

3D structure databases

ProteinModelPortaliB1M5H7
SMRiB1M5H7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI Bacteria
COG1206 LUCA
HOGENOMiHOG000252054
KOiK04094
OMAiRFGPMKP
OrthoDBiPOG091H00B3

Family and domain databases

Gene3Di3.50.50.60, 2 hits
HAMAPiMF_01037 TrmFO, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002218 MnmG-rel
IPR020595 MnmG-rel_CS
IPR004417 TrmFO
PANTHERiPTHR11806 PTHR11806, 1 hit
PTHR11806:SF2 PTHR11806:SF2, 1 hit
PfamiView protein in Pfam
PF01134 GIDA, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00137 gid_trmFO, 1 hit
PROSITEiView protein in PROSITE
PS01281 GIDA_2, 1 hit

Sequencei

Sequence statusi: Complete.

B1M5H7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQIIHIVGG GLAGSEAAWQ VAEAGHRAVI HEMRPVRGTE AHRTDGLAEL
60 70 80 90 100
VCSNSFRSDD PEGNAVGLLH QEMRSLGSLI MRAADTNQVP AGGALAVDRE
110 120 130 140 150
GFSAAVTRAL EQHPNVTLVR GEVEGLPPEA WGPCIVATGP LTAPALAEGI
160 170 180 190 200
RGLTGAESLA FFDAIAPIVH RDSIDMDVAW FQSRYDKPGP GGTGADYLNC
210 220 230 240 250
PMSREQYDTF VAALVAGEKI GFKQWEGTPY FDGCLPVEVM AERGPETLRH
260 270 280 290 300
GPMKPVGLTN PRDPLVKPCA IVQLRQDNAL GTLYNMVGFQ TKLTYSEQVR
310 320 330 340 350
IFRMIPGLER AEFARLGGLH RNTYLDSPRL LDATLRLRAR PSLRFAGQIT
360 370 380 390 400
GCEGYVESAA VGLMAGRFAV AEAAGRPLAP LPQSTALGAL IAHITGGHLM
410 420 430 440 450
ADGEANAPRS FQPMNVNFGL FPPLERAPRN ETGRRLRGPE KAALKKRALT
460 470
DRARADLALW LEGARDGAAR PAAAE
Length:475
Mass (Da):50,894
Last modified:April 29, 2008 - v1
Checksum:i86C80D3CB384142F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA Translation: ACB23568.1
RefSeqiWP_012318556.1, NC_010505.1

Genome annotation databases

EnsemblBacteriaiACB23568; ACB23568; Mrad2831_1573
GeneIDi6137601
KEGGimrd:Mrad2831_1573

Similar proteinsi

Entry informationi

Entry nameiTRMFO_METRJ
AccessioniPrimary (citable) accession number: B1M5H7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: April 29, 2008
Last modified: May 23, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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