B1M3X3 (UREF_METRJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease accessory protein UreF

Gene names

Name:	ureF
Ordered Locus Names:	Mrad2831_2866

Organism

Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP]

Taxonomic identifier

426355 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity. HAMAP MF_01385
Subunit structure	UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01385.
Sequence similarities	Belongs to the UreF family.

Ontologies

Keywords
Biological process	Nickel insertion
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Urease accessory protein UreF HAMAP MF_01385		PRO_0000344136

Sequences

	Sequence		Length	Mass (Da)
	B1M3X3 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: B0B11FE896FAE942	FASTA	229	23,689
	10 20 30 40 50 60 MPPAEALEAL RLMAWLSPGY PVGAYAYSHG LEWAVEAGDV RDEASLTAWL RDVCERGALR 70 80 90 100 110 120 NDLILAAQAH AAARTSDGAA LVAVNDLALA LAPSRELHLE TSQQGRSFLD ATRGAWPCDA 130 140 150 160 170 180 LAALAEELPG PVAYPVAVGL AAGAHGMAPE PVLAAYGLAF LQNLVSAALR AAPVGQAAGT 190 200 210 220 RVVAALAPRL AGLAEAAAAS DLDALGAATF RLDLGSFRHE TQYSRIFRS

References

[1]

"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C.J., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001001 Genomic DNA. Translation: ACB24850.1.
RefSeq	YP_001755533.1. NC_010505.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B1M3X3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6138910.
GenomeReviews	Gene locus Mrad2831_2866 in contig CP001001_GR.
KEGG	mrd:Mrad2831_2866.
PATRIC	22574096. VBIMetRad70578_2931.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG709295.
OMA	WLSPAFP.
ProtClustDB	CLSK2331468.
Family and domain databases
HAMAP	MF_01385. UreF. [Tree]
InterPro	IPR002639. UreF. [Graphical view]
KO	K03188.
Pfam	PF01730. UreF. 1 hit. [Graphical view]
PIRSF	PIRSF009467. Ureas_acces_UreF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UREF_METRJ

Accession

Primary (citable) accession number: B1M3X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program