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B1M335 (SYR_METRJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arginine--tRNA ligase

EC=6.1.1.19
Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS
Gene names
Name:argS
Ordered Locus Names:Mrad2831_2767
OrganismMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP]
Taxonomic identifier426355 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length583 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00123

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00123.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

arginine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 583583Arginine--tRNA ligase HAMAP-Rule MF_00123
PRO_1000198922

Regions

Motif130 – 14011"HIGH" region HAMAP-Rule MF_00123

Sequences

Sequence LengthMass (Da)Tools
B1M335 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 95D687A5723AEDB2

FASTA58363,927
        10         20         30         40         50         60 
MNIFALFEAR VRDAVEALTR AGHLPEGLDL GRVVVEPPRD ASHGDLATNA ALVLAKEARA 

        70         80         90        100        110        120 
NPKALGESLA AELRADPRIV EASVAGPGFI NLRLDPAIFH AVVRDALEAP EAFGRARLPG 

       130        140        150        160        170        180 
GPINVEYVSA NPTGPMHVGH GRGAVFGDAL CNLLVAAGRQ VTREYYINDA GVQVDVLARS 

       190        200        210        220        230        240 
AFQRYREALG EPFAIAEGLY PGDYLKPVGA RLAETHGRAL LDKPEAEWLP TVRETALGMM 

       250        260        270        280        290        300 
MDMIRADLAA IGIRHDVFFS ERTLQESGAV KALLAELRAK GLVYEGRLPP PKGQLPEDWE 

       310        320        330        340        350        360 
DREQTLFRTK EFGDDSDRPL LKSDGSYTYF ASDIAYHRAK YLAGATELID VLGADHGGYV 

       370        380        390        400        410        420 
KRMQAAVKAV SDGKATLDVK LCQLVRLLRA GEPVKMSKRA GEFVTLRDVI DEVGRDAIRF 

       430        440        450        460        470        480 
MMLYRKNDAT LDFDLAKVVE QSKDNPVFYV QYAHARVRSV QRQAREAFPD ADLSVAALAR 

       490        500        510        520        530        540 
EADLAVLSDP GEIEMMRLIA QYPRVLESAA LAHEPHRIAF HLYELASALH SFWNKGKDLP 

       550        560        570        580 
QLRFVNATDR KSTWARLALV EALRSTLAAG LAILGVTAPD EMR 

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Marx C.J., Richardson P.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001001 Genomic DNA. Translation: ACB24751.1.
RefSeqYP_001755434.1. NC_010505.1.

3D structure databases

ProteinModelPortalB1M335.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING426355.Mrad2831_2767.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB24751; ACB24751; Mrad2831_2767.
GeneID6138810.
KEGGmrd:Mrad2831_2767.
PATRIC22573892. VBIMetRad70578_2830.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0018.
HOGENOMHOG000247214.
KOK01887.
OMANPNGPLH.
OrthoDBEOG6JB13C.
ProtClustDBPRK01611.

Enzyme and pathway databases

BioCycMRAD426355:GJB5-2800-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPMF_00123. Arg_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERPTHR11956. PTHR11956. 1 hit.
PfamPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSPR01038. TRNASYNTHARG.
SMARTSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsTIGR00456. argS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYR_METRJ
AccessionPrimary (citable) accession number: B1M335
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 29, 2008
Last modified: April 16, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries