B1M0P4 (B1M0P4_METRJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Mrad2831_1005

Organism

Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP]

Taxonomic identifier

426355 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	894 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B1M0P4 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: CD658F62E1AABEAE	FASTA	894	97,590
	10 20 30 40 50 60 MKVVVVESPA KAKTINKYLG RDYEVIASFG HIRDLPPKDG SVDPEQDFRM VWELADRGAS 70 80 90 100 110 120 RVSEIAKAVK GADKLILATD PDREGEAISW HVLEALTARK ALKGIPVERV TFNAITKASV 130 140 150 160 170 180 EQAMRKPREI DQALVDAYLA RRALDYLVGF NLSPVLWRKL PGARSAGRVQ SVALRLVVER 190 200 210 220 230 240 EMEIERFKPR EYWTLIATLT TADGATFEAR LVGADGKRIQ RLDVGTGEEA AAFRRDLELA 250 260 270 280 290 300 TFQVASVEAK PAKRHPQPPF TTSTLQQEAS RKLGMAPAQT MRVAQRLYEG VDIGGETVGI 310 320 330 340 350 360 ITYMRTDGVD MAPEAIQDAR RVIGKEFGDR YVPDVPRKYS VKAKNAQEAH EAVRPTDMGR 370 380 390 400 410 420 LPKTVARHLE PEQARLYELI WTRTMASQME SAELERTTVD VTAKVGPRTI DLRATGQVVK 430 440 450 460 470 480 FDGFLTLYQE GKDDEEDEES RRLPPMKAGD PLTRERISAT QHFTEPPPRY SEASLVKRME 490 500 510 520 530 540 ELGIGRPSTY AAVLQTLRDR EYVKIEKKRL QPEDKGRLVT GFLESFFRRY VEYDFTAGLE 550 560 570 580 590 600 EQLDRVSNAE IDWRAVLRDF WRDFSAAIGE TKELRVADVL EALNGLLGPH IFPDKGDGSN 610 620 630 640 650 660 PRTCPTCGSG QLSLKLGKFG AFIGCSNYPE CKYTRQLSAT GIDGDGDGSS AEGGQPGVRV 670 680 690 700 710 720 LGDDPATGLP VTLRDGRFGP FLQLGEASTE KGAEKPKRSS LPKGLSPSDV DLNKALALLA 730 740 750 760 770 780 LPREVARHPE TGEPILANLG RFGPYVQHGK TYANLGKDDD VLEIGANRAI DLIVAKEQGG 790 800 810 820 830 840 GRGRLAADPG RPLGKDEASG RDLVVKAGKY GPYVTDGEVN ATLPKTMSAE ALTLDEAIAL 850 860 870 880 890 VNAKRAAGGG KPAKRGAARK SSARAASGDK PAAKKTATKK PAAKKAATKK SSSG

References

[1]

"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C.J., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001001 Genomic DNA. Translation: ACB23014.1.
RefSeq	YP_001753697.1. NC_010505.1.
3D structure databases
ProteinModelPortal	B1M0P4.
ModBase	Search...
Protein-protein interaction databases
STRING	B1M0P4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6137022.
GenomeReviews	Gene locus Mrad2831_1005 in contig CP001001_GR.
KEGG	mrd:Mrad2831_1005.
PATRIC	22570274. VBIMetRad70578_1046.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1M0P4_METRJ

Accession

Primary (citable) accession number: B1M0P4

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)