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B1M0I6

- SYE2_METRJ

UniProt

B1M0I6 - SYE2_METRJ

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Protein
Glutamate--tRNA ligase 2
Gene
gltX2, Mrad2831_5563
Organism
Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei242 – 2421ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMRAD426355:GJB5-5627-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2 (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name:
GluRS 2
Gene namesi
Name:gltX2
Ordered Locus Names:Mrad2831_5563
OrganismiMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Taxonomic identifieri426355 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000006589: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Glutamate--tRNA ligase 2UniRule annotation
PRO_0000367712Add
BLAST

Proteomic databases

PRIDEiB1M0I6.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi426355.Mrad2831_5563.

Structurei

3D structure databases

ProteinModelPortaliB1M0I6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi8 – 1811"HIGH" regionUniRule annotation
Add
BLAST
Motifi239 – 2435"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252721.
KOiK01885.
OMAiRIALFNW.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1M0I6-1 [UniParc]FASTAAdd to Basket

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MTLVRFAPSP TGYLHIGNAR PALLNALFAR RTGGRFLLRL DDTDAERSTE    50
AFAEAIGEDL AWLGIVPDLF ARQSARTAQH DAAADRLRAA GRLYPCYETP 100
EELERRRRRQ LGRGQPPIYD RAALRLTGDE RAALEAEGRR PHWRFLLEAR 150
TVGWDDLVRG PAHVDCASLS DPVLIRADGS YLYTLPSVVD DADLGITHVI 200
RGEDHVTNTG VQVQIFEALG AAVPVFGHHN LLTTADGEGL SKRLGHLSLR 250
GLREAGYEPA AVRSLAVLTG SAESVRAVPD LDTLAGLVDL GEISRAPARF 300
DPAELDGLNA RLIHAMPYRE AAARLADLGI PADRAEAFWL AVRANLSRVP 350
EAAPWWRVVT GPVEPVLTEP AVIAAAVESL PPEPFGPETW NAWTTEIRTR 400
TGAKGRGLFM PLRLALTGLE HGPDLAGLLP LIGRERAARR LSGAAA 446
Length:446
Mass (Da):48,358
Last modified:April 29, 2008 - v1
Checksum:iB09056FD871CD5FB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001001 Genomic DNA. Translation: ACB27508.1.
RefSeqiYP_001758191.1. NC_010505.1.

Genome annotation databases

EnsemblBacteriaiACB27508; ACB27508; Mrad2831_5563.
GeneIDi6141637.
KEGGimrd:Mrad2831_5563.
PATRICi22579544. VBIMetRad70578_5627.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001001 Genomic DNA. Translation: ACB27508.1 .
RefSeqi YP_001758191.1. NC_010505.1.

3D structure databases

ProteinModelPortali B1M0I6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 426355.Mrad2831_5563.

Proteomic databases

PRIDEi B1M0I6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB27508 ; ACB27508 ; Mrad2831_5563 .
GeneIDi 6141637.
KEGGi mrd:Mrad2831_5563.
PATRICi 22579544. VBIMetRad70578_5627.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252721.
KOi K01885.
OMAi RIALFNW.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci MRAD426355:GJB5-5627-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marx C.J., Richardson P.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Entry informationi

Entry nameiSYE2_METRJ
AccessioniPrimary (citable) accession number: B1M0I6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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