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B1M0I6

- SYE2_METRJ

UniProt

B1M0I6 - SYE2_METRJ

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Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei242 – 2421ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMRAD426355:GJB5-5627-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 2UniRule annotation
Short name:
GluRS 2UniRule annotation
Gene namesi
Name:gltX2UniRule annotation
Ordered Locus Names:Mrad2831_5563
OrganismiMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Taxonomic identifieri426355 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000006589: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Glutamate--tRNA ligase 2PRO_0000367712Add
BLAST

Proteomic databases

PRIDEiB1M0I6.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi426355.Mrad2831_5563.

Structurei

3D structure databases

ProteinModelPortaliB1M0I6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi8 – 1811"HIGH" regionAdd
BLAST
Motifi239 – 2435"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252721.
KOiK01885.
OMAiRIALFNW.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1M0I6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLVRFAPSP TGYLHIGNAR PALLNALFAR RTGGRFLLRL DDTDAERSTE
60 70 80 90 100
AFAEAIGEDL AWLGIVPDLF ARQSARTAQH DAAADRLRAA GRLYPCYETP
110 120 130 140 150
EELERRRRRQ LGRGQPPIYD RAALRLTGDE RAALEAEGRR PHWRFLLEAR
160 170 180 190 200
TVGWDDLVRG PAHVDCASLS DPVLIRADGS YLYTLPSVVD DADLGITHVI
210 220 230 240 250
RGEDHVTNTG VQVQIFEALG AAVPVFGHHN LLTTADGEGL SKRLGHLSLR
260 270 280 290 300
GLREAGYEPA AVRSLAVLTG SAESVRAVPD LDTLAGLVDL GEISRAPARF
310 320 330 340 350
DPAELDGLNA RLIHAMPYRE AAARLADLGI PADRAEAFWL AVRANLSRVP
360 370 380 390 400
EAAPWWRVVT GPVEPVLTEP AVIAAAVESL PPEPFGPETW NAWTTEIRTR
410 420 430 440
TGAKGRGLFM PLRLALTGLE HGPDLAGLLP LIGRERAARR LSGAAA
Length:446
Mass (Da):48,358
Last modified:April 29, 2008 - v1
Checksum:iB09056FD871CD5FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA. Translation: ACB27508.1.
RefSeqiYP_001758191.1. NC_010505.1.

Genome annotation databases

EnsemblBacteriaiACB27508; ACB27508; Mrad2831_5563.
GeneIDi6141637.
KEGGimrd:Mrad2831_5563.
PATRICi22579544. VBIMetRad70578_5627.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA. Translation: ACB27508.1 .
RefSeqi YP_001758191.1. NC_010505.1.

3D structure databases

ProteinModelPortali B1M0I6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 426355.Mrad2831_5563.

Proteomic databases

PRIDEi B1M0I6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB27508 ; ACB27508 ; Mrad2831_5563 .
GeneIDi 6141637.
KEGGi mrd:Mrad2831_5563.
PATRICi 22579544. VBIMetRad70578_5627.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252721.
KOi K01885.
OMAi RIALFNW.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci MRAD426355:GJB5-5627-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marx C.J., Richardson P.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Entry informationi

Entry nameiSYE2_METRJ
AccessioniPrimary (citable) accession number: B1M0I6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: November 26, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3