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Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei242ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 2UniRule annotation
Short name:
GluRS 2UniRule annotation
Gene namesi
Name:gltX2UniRule annotation
Ordered Locus Names:Mrad2831_5563
OrganismiMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1)
Taxonomic identifieri426355 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000006589 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003677121 – 446Glutamate--tRNA ligase 2Add BLAST446

Proteomic databases

PRIDEiB1M0I6

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi426355.Mrad2831_5563

Structurei

3D structure databases

ProteinModelPortaliB1M0I6
SMRiB1M0I6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi8 – 18"HIGH" regionUniRule annotationAdd BLAST11
Motifi239 – 243"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252721
KOiK01885
OMAiLYPCYET
OrthoDBiPOG091H021W

Family and domain databases

Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B1M0I6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLVRFAPSP TGYLHIGNAR PALLNALFAR RTGGRFLLRL DDTDAERSTE
60 70 80 90 100
AFAEAIGEDL AWLGIVPDLF ARQSARTAQH DAAADRLRAA GRLYPCYETP
110 120 130 140 150
EELERRRRRQ LGRGQPPIYD RAALRLTGDE RAALEAEGRR PHWRFLLEAR
160 170 180 190 200
TVGWDDLVRG PAHVDCASLS DPVLIRADGS YLYTLPSVVD DADLGITHVI
210 220 230 240 250
RGEDHVTNTG VQVQIFEALG AAVPVFGHHN LLTTADGEGL SKRLGHLSLR
260 270 280 290 300
GLREAGYEPA AVRSLAVLTG SAESVRAVPD LDTLAGLVDL GEISRAPARF
310 320 330 340 350
DPAELDGLNA RLIHAMPYRE AAARLADLGI PADRAEAFWL AVRANLSRVP
360 370 380 390 400
EAAPWWRVVT GPVEPVLTEP AVIAAAVESL PPEPFGPETW NAWTTEIRTR
410 420 430 440
TGAKGRGLFM PLRLALTGLE HGPDLAGLLP LIGRERAARR LSGAAA
Length:446
Mass (Da):48,358
Last modified:April 29, 2008 - v1
Checksum:iB09056FD871CD5FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA Translation: ACB27508.1
RefSeqiWP_012322451.1, NC_010505.1

Genome annotation databases

EnsemblBacteriaiACB27508; ACB27508; Mrad2831_5563
GeneIDi6141637
KEGGimrd:Mrad2831_5563
PATRICifig|426355.14.peg.5627

Similar proteinsi

Entry informationi

Entry nameiSYE2_METRJ
AccessioniPrimary (citable) accession number: B1M0I6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: May 23, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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