B1M0D1 (FTHS_METRJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	Mrad2831_4072

Organism

Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP]

Taxonomic identifier

426355 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium › Methylobacterium radiotolerans

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_1000196816
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1M0D1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 809722BF98FD83CA	FASTA	557	59,349
	10 20 30 40 50 60 MPSDIEIARA ATLKPIAQVA EKLGIPDDAL HNYGKHIAKI DHGYIAGLES RKPGKLVLVT 70 80 90 100 110 120 AISPTPAGEG KTTTTVGLGD ALNRIGEKTM ICLREPSLGP CFGMKGGAAG GGKAQVVPME 130 140 150 160 170 180 QINLHFTGDF HAITSAHSLA AALIDNHVYW ANELNIDVRR IHWRRVVDMN DRALRAITQS 190 200 210 220 230 240 LGGVANGFPR EDGFDITVAS EVMAVFCLAK DLADLEERLG RIVIAETRDR KLVTLKDVKA 250 260 270 280 290 300 TGAMTVLLKD ALQPNLVQTL EGNPALIHGG PFANIAHGCN SVIATRAGLR LADYTVTEAG 310 320 330 340 350 360 FGADLGAEKF LDIKCRQAGL TPSAVVVVAT IRALKMHGGV DKKNLGAENI DALEKGFANL 370 380 390 400 410 420 ARHVTNLRGF GLPVVVGVNH FHADTEAEHA KLKELCRERL DVEAITCRHW AEGGAGAEEL 430 440 450 460 470 480 ARAVVKLSQA EPAPIRHAYE TESRLTDKIR AIATKLYGAA DIQIETKAAG KLATFEKDGY 490 500 510 520 530 540 GHLPICMAKT QYSFSTDPTL MGAPEGHIVG VRDVRLSAGA GFVVAICGEI MTMPGLPRVP 550 AADTIRLDAN GQIDGLF

References

[1]

"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C.J., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001001 Genomic DNA. Translation: ACB26042.1.
RefSeq	YP_001756725.1. NC_010505.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	426355.Mrad2831_4072.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB26042; ACB26042; Mrad2831_4072.
GeneID	6140130.
KEGG	mrd:Mrad2831_4072.
PATRIC	22576588. VBIMetRad70578_4164.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	EIMAVLC.
ProtClustDB	CLSK2330235.
Enzyme and pathway databases
BioCyc	MRAD426355:GJB5-4120-MONOMER.
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_METRJ

Accession

Primary (citable) accession number: B1M0D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program