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B1M0D1 (FTHS_METRJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:Mrad2831_4072
OrganismMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP]
Taxonomic identifier426355 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length557 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 557557Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_1000196816

Regions

Nucleotide binding65 – 728ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1M0D1 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 809722BF98FD83CA

FASTA55759,349
        10         20         30         40         50         60 
MPSDIEIARA ATLKPIAQVA EKLGIPDDAL HNYGKHIAKI DHGYIAGLES RKPGKLVLVT 

        70         80         90        100        110        120 
AISPTPAGEG KTTTTVGLGD ALNRIGEKTM ICLREPSLGP CFGMKGGAAG GGKAQVVPME 

       130        140        150        160        170        180 
QINLHFTGDF HAITSAHSLA AALIDNHVYW ANELNIDVRR IHWRRVVDMN DRALRAITQS 

       190        200        210        220        230        240 
LGGVANGFPR EDGFDITVAS EVMAVFCLAK DLADLEERLG RIVIAETRDR KLVTLKDVKA 

       250        260        270        280        290        300 
TGAMTVLLKD ALQPNLVQTL EGNPALIHGG PFANIAHGCN SVIATRAGLR LADYTVTEAG 

       310        320        330        340        350        360 
FGADLGAEKF LDIKCRQAGL TPSAVVVVAT IRALKMHGGV DKKNLGAENI DALEKGFANL 

       370        380        390        400        410        420 
ARHVTNLRGF GLPVVVGVNH FHADTEAEHA KLKELCRERL DVEAITCRHW AEGGAGAEEL 

       430        440        450        460        470        480 
ARAVVKLSQA EPAPIRHAYE TESRLTDKIR AIATKLYGAA DIQIETKAAG KLATFEKDGY 

       490        500        510        520        530        540 
GHLPICMAKT QYSFSTDPTL MGAPEGHIVG VRDVRLSAGA GFVVAICGEI MTMPGLPRVP 

       550 
AADTIRLDAN GQIDGLF 

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Marx C.J., Richardson P.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001001 Genomic DNA. Translation: ACB26042.1.
RefSeqYP_001756725.1. NC_010505.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING426355.Mrad2831_4072.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB26042; ACB26042; Mrad2831_4072.
GeneID6140130.
KEGGmrd:Mrad2831_4072.
PATRIC22576588. VBIMetRad70578_4164.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMACGEIMTM.
OrthoDBEOG6PCPSP.
ProtClustDBCLSK2330235.

Enzyme and pathway databases

BioCycMRAD426355:GJB5-4120-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_METRJ
AccessionPrimary (citable) accession number: B1M0D1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: April 16, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways