B1LYS1 (LPXK_METRJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	Mrad2831_0888

Organism

Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP]

Taxonomic identifier

426355 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_1000134747
Regions
	Nucleotide binding	52 – 59	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B1LYS1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 70C90E8F82558C02	FASTA	326	33,463
	10 20 30 40 50 60 MRPPAFWAAG PDHPAARGLG PLGAVYGALA ARRMDRPGAR AGCPVLCLGN FTLGGAGKTP 70 80 90 100 110 120 AALAVAALVA ELGAAPAFLS RGYGGRLAGP VRVDPAHHAA AEVGDEPLLL ARRAPAVVAR 130 140 150 160 170 180 DRPAGAALCR SLGADVIVMD DGLQNPSLAK DLSLAVVDGP AGLGNGLPFP AGPLRAPLAR 190 200 210 220 230 240 QWPHVGGLIV IGNGAGGDAV ARAAERRGLP VHGARLVREA DDLAGRRCLA FAGIGRPEKF 250 260 270 280 290 300 YATLAEAGAV IVGTRPYPDH HPYRARELAA LAEAARRLDA ELVTTEKDAV RLPRAFAAGV 310 320 RVLRVRLAFD DAEALRRQIR GALGAP

References

[1]

"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C.J., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001001 Genomic DNA. Translation: ACB22898.1.
RefSeq	YP_001753581.1. NC_010505.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B1LYS1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6136904.
GenomeReviews	Gene locus Mrad2831_0888 in contig CP001001_GR.
KEGG	mrd:Mrad2831_0888.
PATRIC	22570028. VBIMetRad70578_0924.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	CLSK2331442.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_METRJ

Accession

Primary (citable) accession number: B1LYS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program