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B1LXZ1 (DDL_METRJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Mrad2831_2353
OrganismMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP]
Taxonomic identifier426355 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 307307D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341132

Regions

Domain101 – 301201ATP-grasp
Nucleotide binding127 – 18256ATP By similarity

Sites

Metal binding2511Magnesium or manganese 1 By similarity
Metal binding2681Magnesium or manganese 1 By similarity
Metal binding2681Magnesium or manganese 2 By similarity
Metal binding2701Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B1LXZ1 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: E05ADD98B4BDBB3F

FASTA30732,635
        10         20         30         40         50         60 
MSKHVAVLLG GTSAEREVSL NSGKACADAL EGEGYRVTRV DVGPDIASVL TALRPDAAFN 

        70         80         90        100        110        120 
ALHGPDGEDG TIQGLLEILK IPYTHSGVLA SALAMNKERA KTVMRAAGVD VPEGRIVNRR 

       130        140        150        160        170        180 
EAARTHPLPP PYVVKPIAEG SSVGVIIVRD GRSHPPQILA SEEWTFGEQV LAEPYIAGRE 

       190        200        210        220        230        240 
LTCGVMGDKA LGVIEVKAAT GDWYDYDAKY APGGSVHVLP AELKPNVYQR VQELSLTAHQ 

       250        260        270        280        290        300 
ALGCRGVSRA DLRYDDTPGG TGLLVVLEVN TQPGMTQTSL VPEMAAHAGL SFGELVRWMV 


EDASLNR 

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Marx C.J., Richardson P.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001001 Genomic DNA. Translation: ACB24348.1.
RefSeqYP_001755031.1. NC_010505.1.

3D structure databases

ProteinModelPortalB1LXZ1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING426355.Mrad2831_2353.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB24348; ACB24348; Mrad2831_2353.
GeneID6138385.
KEGGmrd:Mrad2831_2353.
PATRIC22573050. VBIMetRad70578_2420.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycMRAD426355:GJB5-2375-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_METRJ
AccessionPrimary (citable) accession number: B1LXZ1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: April 29, 2008
Last modified: February 19, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways