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B1LWN4

- LIPA_METRJ

UniProt

B1LWN4 - LIPA_METRJ

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Protein
Lipoyl synthase
Gene
lipA, Mrad2831_2176
Organism
Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi90 – 901Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi97 – 971Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMRAD426355:GJB5-2198-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Mrad2831_2176
OrganismiMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Taxonomic identifieri426355 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000006589: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Lipoyl synthaseUniRule annotation
PRO_1000099613Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi426355.Mrad2831_2176.

Structurei

3D structure databases

ProteinModelPortaliB1LWN4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiPEEPYNT.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B1LWN4-1 [UniParc]FASTAAdd to Basket

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MAVVLDTLKN DPRPVRLRHP EKAHRPDQPV QAKKPDWIRV KAPGSRAWSE    50
TQKIVREHGL VTVCEEAGCP NIGECWEKRH ATFMIMGDTC TRACAFCNVR 100
TGLPDALDLG EPDKIADSVA KLGLHHVVIT SVDRDDLRDG GAEHFARTIA 150
AIRRASPGTT VEILTPDFLR KDGALEVVVA AKPDVFNHNL ETVPAKYLTV 200
RPGARYFHSV RLLQRVKELD PAIFTKSGIM VGLGEERNEV LQLMDDLRSA 250
DVDFLTIGQY LQPSKKHHEV VRFVPPDEFK AYETTAYAKG FLLVSATPLT 300
RSSHHAGEDF ARLQAARLAK LSPALSA 327
Length:327
Mass (Da):36,245
Last modified:April 29, 2008 - v1
Checksum:iAA09B4FF34385F53
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001001 Genomic DNA. Translation: ACB24171.1.
RefSeqiYP_001754854.1. NC_010505.1.

Genome annotation databases

EnsemblBacteriaiACB24171; ACB24171; Mrad2831_2176.
GeneIDi6138208.
KEGGimrd:Mrad2831_2176.
PATRICi22572684. VBIMetRad70578_2237.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001001 Genomic DNA. Translation: ACB24171.1 .
RefSeqi YP_001754854.1. NC_010505.1.

3D structure databases

ProteinModelPortali B1LWN4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 426355.Mrad2831_2176.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB24171 ; ACB24171 ; Mrad2831_2176 .
GeneIDi 6138208.
KEGGi mrd:Mrad2831_2176.
PATRICi 22572684. VBIMetRad70578_2237.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi PEEPYNT.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci MRAD426355:GJB5-2198-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marx C.J., Richardson P.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Entry informationi

Entry nameiLIPA_METRJ
AccessioniPrimary (citable) accession number: B1LWN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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