Reviewed, UniProtKB/Swiss-Prot B1LUJ9 (SYE1_METRJ)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Mrad2831_2022

Organism

Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP]

Taxonomic identifier

426355 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_1000090089
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00022
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1LUJ9-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 9F832F1406F04989	FASTA	475	51,722
	10 20 30 40 50 60 MSSAVVTRFA PSPTGYLHIG GARTALFNWL YARHTGGKML LRIEDTDRER STKGAIDAIL 70 80 90 100 110 120 DGLSWLGLDW DGDVVFQFAR AERHRAVAEE LLAAGRAYHC YATAEELAQM RETARAEGRA 130 140 150 160 170 180 PRYDGRWRDR DPSEAPAGVK PVIRLRAPIE GETVVEDAVQ GRVTWANKDL DDLVLLRSDG 190 200 210 220 230 240 TPTYMLAVVV DDHDMGVTQI IRGDDHLTNA ARQSQIFSAL GWDVPRMAHI PLIHGADGAK 250 260 270 280 290 300 LSKRHGALGV EAYRDLGYLP AALRNYLVRL GWSHGDQEVF STEEMVAAFD LGAVGRSAAR 310 320 330 340 350 360 FDFAKLANLN GLYIRTSADA DLVAAIETIL PNVGPERGLS APLQPDLKDK LIQAMPGLKE 370 380 390 400 410 420 RAKTLIELLD SAYYLYAQRP LALDDKARAL LSDEGRGRLA GVRPVLEALP DWSAASTEGA 430 440 450 460 470 VRQYAESAGC KLGQVAQPLR AALTGRTTSP PLFDVMAVLG REETLARLGD QAPQG

References

[1]

"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C.J., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001001 Genomic DNA. Translation: ACB24017.1.
RefSeq	YP_001754700.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6138051.
GenomeReviews	Gene locus Mrad2831_2022 in contig CP001001_GR.
KEGG	mrd:Mrad2831_2022.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MAHIPLI.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_METRJ

Accession

Primary (citable) accession number: B1LUJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)