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Protein

Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase

Gene

lpxA

Organism
Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine.UniRule annotation

Pathway: lipid IV(A) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (Mrad2831_2875), UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC), UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Lipid-A-disaccharide synthase (lpxB), Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciMRAD426355:GJB5-3474-MONOMER.
UniPathwayiUPA00359; UER00477.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseUniRule annotation (EC:2.3.1.129UniRule annotation)
Short name:
UDP-N-acetylglucosamine acyltransferaseUniRule annotation
Gene namesi
Name:lpxAUniRule annotation
Ordered Locus Names:Mrad2831_3436
OrganismiMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Taxonomic identifieri426355 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000006589 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 272272Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferasePRO_1000122715Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi426355.Mrad2831_3436.

Structurei

3D structure databases

ProteinModelPortaliB1LTP4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transferase hexapeptide repeat family. LpxA subfamily.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1043.
HOGENOMiHOG000294326.
KOiK00677.
OMAiINTHIAH.
OrthoDBiEOG6F81P1.

Family and domain databases

Gene3Di1.20.1180.10. 1 hit.
HAMAPiMF_00387. LpxA.
InterProiIPR029098. Acetyltransf_C.
IPR001451. Hexapep_transf.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 2 hits.
[Graphical view]
PIRSFiPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01852. lipid_A_lpxA. 1 hit.

Sequencei

Sequence statusi: Complete.

B1LTP4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPAIHPSA VIESGARIGD GARIGPFCHV GPEVVLGADC ELISHVVLAG
60 70 80 90 100
RTTIGPRTRI FPFASIGHQP QDLKYRGEAS TLTIGADCLI REGVTMNPGT
110 120 130 140 150
SGGGLETLVG DHCTFLANSH VGHDCRVGAH VIFSNNVMLA GHCSVGDYAI
160 170 180 190 200
LGGGAAVIQF ARVGAHAFVG GLSGLENDCI PYGMVLGNRA YLSGLNIIGL
210 220 230 240 250
QRRGFAREDI HALRRAYRLL FAPEGTLMER VEDVAATFES HAAVAEILDF
260 270
IRLGGKRSIC TPREVPTPIS AA
Length:272
Mass (Da):28,560
Last modified:April 29, 2008 - v1
Checksum:iAC689D8B75C302CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA. Translation: ACB25413.1.
RefSeqiWP_012320376.1. NC_010505.1.
YP_001756096.1. NC_010505.1.

Genome annotation databases

EnsemblBacteriaiACB25413; ACB25413; Mrad2831_3436.
GeneIDi23622328.
KEGGimrd:Mrad2831_3436.
PATRICi22575262. VBIMetRad70578_3511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA. Translation: ACB25413.1.
RefSeqiWP_012320376.1. NC_010505.1.
YP_001756096.1. NC_010505.1.

3D structure databases

ProteinModelPortaliB1LTP4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi426355.Mrad2831_3436.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB25413; ACB25413; Mrad2831_3436.
GeneIDi23622328.
KEGGimrd:Mrad2831_3436.
PATRICi22575262. VBIMetRad70578_3511.

Phylogenomic databases

eggNOGiCOG1043.
HOGENOMiHOG000294326.
KOiK00677.
OMAiINTHIAH.
OrthoDBiEOG6F81P1.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00477.
BioCyciMRAD426355:GJB5-3474-MONOMER.

Family and domain databases

Gene3Di1.20.1180.10. 1 hit.
HAMAPiMF_00387. LpxA.
InterProiIPR029098. Acetyltransf_C.
IPR001451. Hexapep_transf.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 2 hits.
[Graphical view]
PIRSFiPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01852. lipid_A_lpxA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marx C.J., Richardson P.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Entry informationi

Entry nameiLPXA_METRJ
AccessioniPrimary (citable) accession number: B1LTP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: May 27, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.