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B1LTD9 (DAPA_METRJ) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:Mrad2831_1890
OrganismMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP]
Taxonomic identifier426355 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 299299Dihydrodipicolinate synthase HAMAP MF_00418
PRO_0000340968

Regions

Region54 – 552Pyruvate binding By similarity

Sites

Active site1671Schiff-base intermediate with substrate By similarity
Binding site1121Pyruvate By similarity
Site1391Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
B1LTD9 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 77356B461932CC81

FASTA29931,981
        10         20         30         40         50         60 
MTEMTGSRLR GSLTALVTPF RDGAFDEAAF RKFVRWQIEQ GSHGLVPTGT TGESPTLTHS 

        70         80         90        100        110        120 
EHDRVVEACI DEAGGRVPVV AGAGSNSTAE AVERAQHAER AGADAVLVVT PYYNKPTQAG 

       130        140        150        160        170        180 
LYAHFKAVND AVGIPIIIYN IPPRSVIDMS VETMARLFEL KNIAGVKDAT AKIDRVSQQR 

       190        200        210        220        230        240 
QAMGDSFIQL SGEDATALGY NAHGGHGCIS VVANVAPRLC ADLQEATLAG DYAKALTLQD 

       250        260        270        280        290 
RLFPLQTGLF AEANPAPVKY ALSRLGHMTD ELRLPLVPVT EPTKRIVDDA LRHAGLLVD

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Marx C.J., Richardson P.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001001 Genomic DNA. Translation: ACB23885.1.
RefSeqYP_001754568.1. NC_010505.1.

3D structure databases

ProteinModelPortalB1LTD9.
SMRB1LTD9. Positions 9-298.
ModBaseSearch...

Protein-protein interaction databases

STRINGB1LTD9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6137919.
GenomeReviewsGene locus Mrad2831_1890 in contig CP001001_GR.
KEGGmrd:Mrad2831_1890.
PATRIC22572102. VBIMetRad70578_1949.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG358848.
OMAHGCISVV.
ProtClustDBPRK03170.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_METRJ
AccessionPrimary (citable) accession number: B1LTD9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: April 29, 2008
Last modified: January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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