B1LT37 (B1LT37_METRJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit B RuleBase RU003363 HAMAP MF_01898
EC=5.99.1.3 RuleBase RU003363 HAMAP MF_01898

Gene names

Name:	gyrB HAMAP MF_01898
Ordered Locus Names:	Mrad2831_0319

Organism

Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP]

Taxonomic identifier

426355 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	814 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. RuleBase RU003363 HAMAP MF_01898
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. SAAS SAAS002288 RuleBase RU003363 HAMAP MF_01898
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity. HAMAP MF_01898
Subcellular location	Cytoplasm By similarity HAMAP MF_01898.
Sequence similarities	Belongs to the type II topoisomerase family. HAMAP MF_01898 RuleBase RU000380

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_01898
Ligand	ATP-binding SAAS SAAS002288 HAMAP MF_01898 RuleBase RU000380 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS002288 HAMAP MF_01898 RuleBase RU000380
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B1LT37 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 6E30762E5C95FECB	FASTA	814	89,708
	10 20 30 40 50 60 MADTPQTEPA VYGAESIRVL KGLDAVRKRP GMYIGDTDDG SGLHHMVYEV VDNAIDEALA 70 80 90 100 110 120 GHADLVTVTL NADGSCTVSD NGRGIPVDIH KEEGVSAAEV IMTQLHAGGK FDQNSYKVSG 130 140 150 160 170 180 GLHGVGVSVV NALSSWLRLR IWRNDKEHAM EFRHGDAVAP LEVIGPGNGR RGTEVTFLPS 190 200 210 220 230 240 TETFTMIEFD YATLEKRLRE LAFLNSGVRI VLTDARHAEK KREELYYEGG IEAFVRYLDR 250 260 270 280 290 300 SRKPIDGMTS PVVVSSERDG IRVEVAFWWN DSFNETVLPF TNNIPQRDGG THMAGFRAAL 310 320 330 340 350 360 TRQVTGYAES SGIAKKEKVS LTGEDCREGL TAVISVQVPD PKFSSQTKDK LVSSEVRPAV 370 380 390 400 410 420 ENVLNEGLST WLEENPSQAR SVMGKVVLAA SAREAARKAR ETVTRKGALD IASLPGKLAD 430 440 450 460 470 480 CQERDPTKCE ILLVEGDSAG GSAKQGRDRT FQAVLPLRGK ILNVERVRAD RMLSSAEIGT 490 500 510 520 530 540 LITALGAGIG RSSTDREGFN PEKLRYHRII IMTDADVDGS HIRTLLLTFF FRQMPELIER 550 560 570 580 590 600 GHLYIAQPPL YKAERGRRAI YLKDERALED YLIDQGTEGA VLRLSSGAEF AGAQLKSLVE 610 620 630 640 650 660 EARTFRSILQ GLHTRYDRSV VEQAVLAGAF DAEAASRAGE AEVLADMTAR RLDAIADEIE 670 680 690 700 710 720 RGWQGESFEG GYRLSRTLRG VRQVSILDAG LITSQEARRL SERADAFREI YGEPATLMRK 730 740 750 760 770 780 SDETVLHGPV ALFEAVMAFG RKGLQLQRYK GLGEMTAQQL WETTLDRDVR SLLQVRVKDV 790 800 810 TDADDLFVKL MGDVVEPRRE FIQENALSVA NLDV

References

[1]

"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C.J., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001001 Genomic DNA. Translation: ACB22343.1.
RefSeq	YP_001753026.1. NC_010505.1.
3D structure databases
ProteinModelPortal	B1LT37.
SMR	B1LT37. Positions 17-387.
ModBase	Search...
Protein-protein interaction databases
STRING	B1LT37.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6136161.
GenomeReviews	Gene locus Mrad2831_0319 in contig CP001001_GR.
KEGG	mrd:Mrad2831_0319.
PATRIC	22568862. VBIMetRad70578_0345.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304889.
OMA	DCSSRDP.
ProtClustDB	PRK14939.
Family and domain databases
HAMAP	MF_01898. GyrB. [Tree]
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011557. GyrB. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR013759. Topo_IIA_B/N_ab. IPR002288. Topo_IIA_B_C. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 2 hits.
KO	K02470.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01059. GyrB. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1LT37_METRJ

Accession

Primary (citable) accession number: B1LT37

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)