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Protein

Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein

Gene

bchL

Organism
Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The L component serves as a unique electron donor to the NB-component of the complex, and binds Mg-ATP.UniRule annotation

Catalytic activityi

Protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O = chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per dimer.UniRule annotation

Pathwayi: bacteriochlorophyll biosynthesis (light-independent)

This protein is involved in the pathway bacteriochlorophyll biosynthesis (light-independent), which is part of Porphyrin-containing compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway bacteriochlorophyll biosynthesis (light-independent) and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45MagnesiumUniRule annotation1
Binding sitei70ATPUniRule annotation1
Metal bindingi126Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi160Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi41 – 46ATPUniRule annotation6
Nucleotide bindingi211 – 212ATPUniRule annotation2
Nucleotide bindingi235 – 237ATPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Bacteriochlorophyll biosynthesis, Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00671.

Names & Taxonomyi

Protein namesi
Recommended name:
Light-independent protochlorophyllide reductase iron-sulfur ATP-binding proteinUniRule annotation (EC:1.3.7.7UniRule annotation)
Short name:
DPOR subunit LUniRule annotation
Short name:
LI-POR subunit LUniRule annotation
Gene namesi
Name:bchLUniRule annotation
Ordered Locus Names:Mrad2831_1845
OrganismiMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Taxonomic identifieri426355 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000006589 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001334411 – 297Light-independent protochlorophyllide reductase iron-sulfur ATP-binding proteinAdd BLAST297

Interactioni

Subunit structurei

Homodimer. Protochlorophyllide reductase is composed of three subunits; BchL, BchN and BchB.UniRule annotation

Protein-protein interaction databases

STRINGi426355.Mrad2831_1845.

Structurei

3D structure databases

ProteinModelPortaliB1LSF9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228825.
KOiK04037.
OMAiTSCNISV.
OrthoDBiPOG091H0230.

Family and domain databases

CDDicd02032. Bchl_like. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00355. ChlL_BchL. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR027417. P-loop_NTPase.
IPR005971. Protochlorophyllide_ATP-bd.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01281. DPOR_bchL. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1LSF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIAIRNPVV ARKEEGSLQV ALDPDLKIET AKVFAVYGKG GIGKSTTSSN
60 70 80 90 100
LSVAFSKLGK RVLQIGCDPK HDSTFTLTKR LAPTVIDALE AVNFHSEELR
110 120 130 140 150
VEDFVVEGYN GVMCVEAGGP PAGTGCGGYV VGQTVKLLKE HHLLEDTDVV
160 170 180 190 200
VFDVLGDVVC GGFASPLQHA DRALIVTAND FDSIFAMNRI VAAIHAKSKN
210 220 230 240 250
YGVRLGGVIA NRSAKTDEID RFNDAVGLKR LAHFPDLDVV RRSRLKKSTL
260 270 280 290
FEMESSPELD AVTAEYMRLA ETLWAGAEPC EAQPMKDRDL FEFLGFD
Length:297
Mass (Da):32,258
Last modified:April 29, 2008 - v1
Checksum:i2BB9BB1B3400EEC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA. Translation: ACB23840.1.
RefSeqiWP_012318826.1. NC_010505.1.

Genome annotation databases

EnsemblBacteriaiACB23840; ACB23840; Mrad2831_1845.
GeneIDi6137874.
KEGGimrd:Mrad2831_1845.
PATRICi22572004. VBIMetRad70578_1900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA. Translation: ACB23840.1.
RefSeqiWP_012318826.1. NC_010505.1.

3D structure databases

ProteinModelPortaliB1LSF9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi426355.Mrad2831_1845.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB23840; ACB23840; Mrad2831_1845.
GeneIDi6137874.
KEGGimrd:Mrad2831_1845.
PATRICi22572004. VBIMetRad70578_1900.

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228825.
KOiK04037.
OMAiTSCNISV.
OrthoDBiPOG091H0230.

Enzyme and pathway databases

UniPathwayiUPA00671.

Family and domain databases

CDDicd02032. Bchl_like. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00355. ChlL_BchL. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR027417. P-loop_NTPase.
IPR005971. Protochlorophyllide_ATP-bd.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01281. DPOR_bchL. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBCHL_METRJ
AccessioniPrimary (citable) accession number: B1LSF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.