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B1LSB2 (B1LSB2_METRJ) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP-Rule MF_02002

EC=6.1.1.5 HAMAP-Rule MF_02002
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP-Rule MF_02002
Gene names
Name:ileS HAMAP-Rule MF_02002
Ordered Locus Names:Mrad2831_1798 EMBL ACB23793.1
OrganismMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP] EMBL ACB23793.1
Taxonomic identifier426355 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length988 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002 SAAS SAAS023585

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. HAMAP-Rule MF_02002

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Motif66 – 7611"HIGH" region By similarity HAMAP-Rule MF_02002
Motif654 – 6585"KMSKS" region By similarity HAMAP-Rule MF_02002

Sites

Binding site6131Aminoacyl-adenylate By similarity HAMAP-Rule MF_02002
Binding site6571ATP By similarity HAMAP-Rule MF_02002

Sequences

Sequence LengthMass (Da)Tools
B1LSB2 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 99A43E3AF21F3147

FASTA988109,463
        10         20         30         40         50         60 
MTAPETAPAR DYSKTLFLPQ TEFPMRAGLP VREPLFIERW KRMDLYGKLR AAGRDRPKFV 

        70         80         90        100        110        120 
LHDGPPYANG NIHIGTALNK ILKDVVVRSQ TALGFDANYV PGWDCHGLPI EWKIEEQYRA 

       130        140        150        160        170        180 
KGRNKDDVPV IEFRQECRTF AAQWLDVQRE EFKRLGVTGD WDHPYATMAF PAEAVIADEL 

       190        200        210        220        230        240 
MKFSMSGQLY RGSKPVMWSV VEKTALAEAE VEYEEHVSDT VFVAFPIRSG AEGDLAAARV 

       250        260        270        280        290        300 
VIWTTTPWTI PGNRAVAYSK KIAYGLYRVT EAPADNWATP GQTYVLADSL AATVFKAARV 

       310        320        330        340        350        360 
EAFARVADVS PSTLAGLTLD HPLAKLGYGF AVPMLDGDHV TDESGTGFVH TAPSHGREDF 

       370        380        390        400        410        420 
ELWMASGRLL RERGIDATIP YTVDADSVLT EAAPGFTGTR VLTAKGEKGD ANKAVIAALT 

       430        440        450        460        470        480 
EAGALIARGV LRHQYPHSWR SKKPVIFRNT PQWFIAMDRP VDSLGNRTLR EVALQGIDDT 

       490        500        510        520        530        540 
QWVPTQGKNR ITGMVANRPD WVVSRQRAWG VPITVFVHRE TAEILRDARV NARIRDAFAA 

       550        560        570        580        590        600 
EGADAWFRDD AAQRFLAPDH DPAAYEKVTD VLDVWFDSGS THAFVLDDPE AFPGLAGVKR 

       610        620        630        640        650        660 
VRDGGRDRVM YLEGSDQHRG WFQSSLLESA GTRGRAPYDI VLTHGFVLDG KGLKMSKSKG 

       670        680        690        700        710        720 
NVVAPQSVIK DSGADILRLW VAASDYTDDL RIGPEIIKTF AETYRKLRNS LRWMLGSLAH 

       730        740        750        760        770        780 
RVPGDDVAYA DMPELERLIL HRLAELDGEI REAYAAFDTK RVVALLNGFM TGDLSSFYFD 

       790        800        810        820        830        840 
VRKDALYCDP VSSVRRRASL QVIDEAFRRV AIWLAPVLAF TAEEAWLDRY PSEDGSVHLQ 

       850        860        870        880        890        900 
TLPETPADWL DPALAERWHR IRRVRRVVTG ALEIERAAKR IGASLEAAPT VYVADPELLA 

       910        920        930        940        950        960 
ALEGCDFADT CITSAITVVA GEGPAEAFRL DEVRGVAVVP SPAEGRKCAR SWRVSPSVGS 

       970        980 
DPDYPEVTPR DAQALREWDA AHPEASAA 

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References

[1]"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Marx C.J., Richardson P.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001001 Genomic DNA. Translation: ACB23793.1.
RefSeqYP_001754476.1. NC_010505.1.

3D structure databases

ProteinModelPortalB1LSB2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING426355.Mrad2831_1798.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB23793; ACB23793; Mrad2831_1798.
GeneID6137827.
KEGGmrd:Mrad2831_1798.
PATRIC22571900. VBIMetRad70578_1848.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAERLMLHQ.
OrthoDBEOG644ZM1.

Enzyme and pathway databases

BioCycMRAD426355:GJB5-1817-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB1LSB2_METRJ
AccessionPrimary (citable) accession number: B1LSB2
Entry history
Integrated into UniProtKB/TrEMBL: April 29, 2008
Last sequence update: April 29, 2008
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)