SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B1LSB2

- B1LSB2_METRJ

UniProt

B1LSB2 - B1LSB2_METRJ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, Mrad2831_1798
Organism
Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei613 – 6131Aminoacyl-adenylate By similarityUniRule annotation
Binding sitei657 – 6571ATP By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationsImported, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciMRAD426355:GJB5-1817-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetase
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mrad2831_1798Imported
OrganismiMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)Imported
Taxonomic identifieri426355 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000006589: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi426355.Mrad2831_1798.

Structurei

3D structure databases

ProteinModelPortaliB1LSB2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" region By similarityUniRule annotation
Add
BLAST
Motifi654 – 6585"KMSKS" region By similarityUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1LSB2-1 [UniParc]FASTAAdd to Basket

« Hide

MTAPETAPAR DYSKTLFLPQ TEFPMRAGLP VREPLFIERW KRMDLYGKLR    50
AAGRDRPKFV LHDGPPYANG NIHIGTALNK ILKDVVVRSQ TALGFDANYV 100
PGWDCHGLPI EWKIEEQYRA KGRNKDDVPV IEFRQECRTF AAQWLDVQRE 150
EFKRLGVTGD WDHPYATMAF PAEAVIADEL MKFSMSGQLY RGSKPVMWSV 200
VEKTALAEAE VEYEEHVSDT VFVAFPIRSG AEGDLAAARV VIWTTTPWTI 250
PGNRAVAYSK KIAYGLYRVT EAPADNWATP GQTYVLADSL AATVFKAARV 300
EAFARVADVS PSTLAGLTLD HPLAKLGYGF AVPMLDGDHV TDESGTGFVH 350
TAPSHGREDF ELWMASGRLL RERGIDATIP YTVDADSVLT EAAPGFTGTR 400
VLTAKGEKGD ANKAVIAALT EAGALIARGV LRHQYPHSWR SKKPVIFRNT 450
PQWFIAMDRP VDSLGNRTLR EVALQGIDDT QWVPTQGKNR ITGMVANRPD 500
WVVSRQRAWG VPITVFVHRE TAEILRDARV NARIRDAFAA EGADAWFRDD 550
AAQRFLAPDH DPAAYEKVTD VLDVWFDSGS THAFVLDDPE AFPGLAGVKR 600
VRDGGRDRVM YLEGSDQHRG WFQSSLLESA GTRGRAPYDI VLTHGFVLDG 650
KGLKMSKSKG NVVAPQSVIK DSGADILRLW VAASDYTDDL RIGPEIIKTF 700
AETYRKLRNS LRWMLGSLAH RVPGDDVAYA DMPELERLIL HRLAELDGEI 750
REAYAAFDTK RVVALLNGFM TGDLSSFYFD VRKDALYCDP VSSVRRRASL 800
QVIDEAFRRV AIWLAPVLAF TAEEAWLDRY PSEDGSVHLQ TLPETPADWL 850
DPALAERWHR IRRVRRVVTG ALEIERAAKR IGASLEAAPT VYVADPELLA 900
ALEGCDFADT CITSAITVVA GEGPAEAFRL DEVRGVAVVP SPAEGRKCAR 950
SWRVSPSVGS DPDYPEVTPR DAQALREWDA AHPEASAA 988
Length:988
Mass (Da):109,463
Last modified:April 29, 2008 - v1
Checksum:i99A43E3AF21F3147
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001001 Genomic DNA. Translation: ACB23793.1.
RefSeqiYP_001754476.1. NC_010505.1.

Genome annotation databases

EnsemblBacteriaiACB23793; ACB23793; Mrad2831_1798.
GeneIDi6137827.
KEGGimrd:Mrad2831_1798.
PATRICi22571900. VBIMetRad70578_1848.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001001 Genomic DNA. Translation: ACB23793.1 .
RefSeqi YP_001754476.1. NC_010505.1.

3D structure databases

ProteinModelPortali B1LSB2.
ModBasei Search...

Protein-protein interaction databases

STRINGi 426355.Mrad2831_1798.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB23793 ; ACB23793 ; Mrad2831_1798 .
GeneIDi 6137827.
KEGGi mrd:Mrad2831_1798.
PATRICi 22571900. VBIMetRad70578_1848.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MRAD426355:GJB5-1817-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marx C.J., Richardson P.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Entry informationi

Entry nameiB1LSB2_METRJ
AccessioniPrimary (citable) accession number: B1LSB2
Entry historyi
Integrated into UniProtKB/TrEMBL: April 29, 2008
Last sequence update: April 29, 2008
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

Similar proteinsi