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B1LSB2

- B1LSB2_METRJ

UniProt

B1LSB2 - B1LSB2_METRJ

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei613 – 6131Aminoacyl-adenylateUniRule annotation
    Binding sitei657 – 6571ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMRAD426355:GJB5-1817-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Mrad2831_1798Imported
    OrganismiMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)Imported
    Taxonomic identifieri426355 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
    ProteomesiUP000006589: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi426355.Mrad2831_1798.

    Structurei

    3D structure databases

    ProteinModelPortaliB1LSB2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi66 – 7611"HIGH" regionUniRule annotationAdd
    BLAST
    Motifi654 – 6585"KMSKS" regionUniRule annotation

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1LSB2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAPETAPAR DYSKTLFLPQ TEFPMRAGLP VREPLFIERW KRMDLYGKLR    50
    AAGRDRPKFV LHDGPPYANG NIHIGTALNK ILKDVVVRSQ TALGFDANYV 100
    PGWDCHGLPI EWKIEEQYRA KGRNKDDVPV IEFRQECRTF AAQWLDVQRE 150
    EFKRLGVTGD WDHPYATMAF PAEAVIADEL MKFSMSGQLY RGSKPVMWSV 200
    VEKTALAEAE VEYEEHVSDT VFVAFPIRSG AEGDLAAARV VIWTTTPWTI 250
    PGNRAVAYSK KIAYGLYRVT EAPADNWATP GQTYVLADSL AATVFKAARV 300
    EAFARVADVS PSTLAGLTLD HPLAKLGYGF AVPMLDGDHV TDESGTGFVH 350
    TAPSHGREDF ELWMASGRLL RERGIDATIP YTVDADSVLT EAAPGFTGTR 400
    VLTAKGEKGD ANKAVIAALT EAGALIARGV LRHQYPHSWR SKKPVIFRNT 450
    PQWFIAMDRP VDSLGNRTLR EVALQGIDDT QWVPTQGKNR ITGMVANRPD 500
    WVVSRQRAWG VPITVFVHRE TAEILRDARV NARIRDAFAA EGADAWFRDD 550
    AAQRFLAPDH DPAAYEKVTD VLDVWFDSGS THAFVLDDPE AFPGLAGVKR 600
    VRDGGRDRVM YLEGSDQHRG WFQSSLLESA GTRGRAPYDI VLTHGFVLDG 650
    KGLKMSKSKG NVVAPQSVIK DSGADILRLW VAASDYTDDL RIGPEIIKTF 700
    AETYRKLRNS LRWMLGSLAH RVPGDDVAYA DMPELERLIL HRLAELDGEI 750
    REAYAAFDTK RVVALLNGFM TGDLSSFYFD VRKDALYCDP VSSVRRRASL 800
    QVIDEAFRRV AIWLAPVLAF TAEEAWLDRY PSEDGSVHLQ TLPETPADWL 850
    DPALAERWHR IRRVRRVVTG ALEIERAAKR IGASLEAAPT VYVADPELLA 900
    ALEGCDFADT CITSAITVVA GEGPAEAFRL DEVRGVAVVP SPAEGRKCAR 950
    SWRVSPSVGS DPDYPEVTPR DAQALREWDA AHPEASAA 988
    Length:988
    Mass (Da):109,463
    Last modified:April 29, 2008 - v1
    Checksum:i99A43E3AF21F3147
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001001 Genomic DNA. Translation: ACB23793.1.
    RefSeqiYP_001754476.1. NC_010505.1.

    Genome annotation databases

    EnsemblBacteriaiACB23793; ACB23793; Mrad2831_1798.
    GeneIDi6137827.
    KEGGimrd:Mrad2831_1798.
    PATRICi22571900. VBIMetRad70578_1848.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001001 Genomic DNA. Translation: ACB23793.1 .
    RefSeqi YP_001754476.1. NC_010505.1.

    3D structure databases

    ProteinModelPortali B1LSB2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 426355.Mrad2831_1798.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB23793 ; ACB23793 ; Mrad2831_1798 .
    GeneIDi 6137827.
    KEGGi mrd:Mrad2831_1798.
    PATRICi 22571900. VBIMetRad70578_1848.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci MRAD426355:GJB5-1817-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831."
      US DOE Joint Genome Institute
      Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
      , Kyrpides N., Mikhailova N., Marx C.J., Richardson P.
      Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27329 / DSM 1819 / JCM 2831Imported.

    Entry informationi

    Entry nameiB1LSB2_METRJ
    AccessioniPrimary (citable) accession number: B1LSB2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 29, 2008
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3