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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei613 – 6131Aminoacyl-adenylateUniRule annotation
Binding sitei657 – 6571ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMRAD426355:GJB5-1817-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotationSAAS annotation (EC:6.1.1.5UniRule annotationSAAS annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mrad2831_1798Imported
OrganismiMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)Imported
Taxonomic identifieri426355 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000006589 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi426355.Mrad2831_1798.

Structurei

3D structure databases

ProteinModelPortaliB1LSB2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionUniRule annotationAdd
BLAST
Motifi654 – 6585"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1LSB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPETAPAR DYSKTLFLPQ TEFPMRAGLP VREPLFIERW KRMDLYGKLR
60 70 80 90 100
AAGRDRPKFV LHDGPPYANG NIHIGTALNK ILKDVVVRSQ TALGFDANYV
110 120 130 140 150
PGWDCHGLPI EWKIEEQYRA KGRNKDDVPV IEFRQECRTF AAQWLDVQRE
160 170 180 190 200
EFKRLGVTGD WDHPYATMAF PAEAVIADEL MKFSMSGQLY RGSKPVMWSV
210 220 230 240 250
VEKTALAEAE VEYEEHVSDT VFVAFPIRSG AEGDLAAARV VIWTTTPWTI
260 270 280 290 300
PGNRAVAYSK KIAYGLYRVT EAPADNWATP GQTYVLADSL AATVFKAARV
310 320 330 340 350
EAFARVADVS PSTLAGLTLD HPLAKLGYGF AVPMLDGDHV TDESGTGFVH
360 370 380 390 400
TAPSHGREDF ELWMASGRLL RERGIDATIP YTVDADSVLT EAAPGFTGTR
410 420 430 440 450
VLTAKGEKGD ANKAVIAALT EAGALIARGV LRHQYPHSWR SKKPVIFRNT
460 470 480 490 500
PQWFIAMDRP VDSLGNRTLR EVALQGIDDT QWVPTQGKNR ITGMVANRPD
510 520 530 540 550
WVVSRQRAWG VPITVFVHRE TAEILRDARV NARIRDAFAA EGADAWFRDD
560 570 580 590 600
AAQRFLAPDH DPAAYEKVTD VLDVWFDSGS THAFVLDDPE AFPGLAGVKR
610 620 630 640 650
VRDGGRDRVM YLEGSDQHRG WFQSSLLESA GTRGRAPYDI VLTHGFVLDG
660 670 680 690 700
KGLKMSKSKG NVVAPQSVIK DSGADILRLW VAASDYTDDL RIGPEIIKTF
710 720 730 740 750
AETYRKLRNS LRWMLGSLAH RVPGDDVAYA DMPELERLIL HRLAELDGEI
760 770 780 790 800
REAYAAFDTK RVVALLNGFM TGDLSSFYFD VRKDALYCDP VSSVRRRASL
810 820 830 840 850
QVIDEAFRRV AIWLAPVLAF TAEEAWLDRY PSEDGSVHLQ TLPETPADWL
860 870 880 890 900
DPALAERWHR IRRVRRVVTG ALEIERAAKR IGASLEAAPT VYVADPELLA
910 920 930 940 950
ALEGCDFADT CITSAITVVA GEGPAEAFRL DEVRGVAVVP SPAEGRKCAR
960 970 980
SWRVSPSVGS DPDYPEVTPR DAQALREWDA AHPEASAA
Length:988
Mass (Da):109,463
Last modified:April 29, 2008 - v1
Checksum:i99A43E3AF21F3147
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA. Translation: ACB23793.1.
RefSeqiWP_012318779.1. NC_010505.1.
YP_001754476.1. NC_010505.1.

Genome annotation databases

EnsemblBacteriaiACB23793; ACB23793; Mrad2831_1798.
KEGGimrd:Mrad2831_1798.
PATRICi22571900. VBIMetRad70578_1848.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001001 Genomic DNA. Translation: ACB23793.1.
RefSeqiWP_012318779.1. NC_010505.1.
YP_001754476.1. NC_010505.1.

3D structure databases

ProteinModelPortaliB1LSB2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi426355.Mrad2831_1798.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB23793; ACB23793; Mrad2831_1798.
KEGGimrd:Mrad2831_1798.
PATRICi22571900. VBIMetRad70578_1848.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciMRAD426355:GJB5-1817-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marx C.J., Richardson P.
    Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27329 / DSM 1819 / JCM 2831Imported.

Entry informationi

Entry nameiB1LSB2_METRJ
AccessioniPrimary (citable) accession number: B1LSB2
Entry historyi
Integrated into UniProtKB/TrEMBL: April 29, 2008
Last sequence update: April 29, 2008
Last modified: April 1, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.