B1LSA1 (CYSD_METRJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 2
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysD
Ordered Locus Names:	Mrad2831_1787

Organism

Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) [Complete proteome] [HAMAP]

Taxonomic identifier

426355 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_0000340203

Sequences

	Sequence		Length	Mass (Da)
	B1LSA1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 4860D824008F5690	FASTA	319	35,938
	10 20 30 40 50 60 MSAALAAAVQ PSAPASAQTA DRLSHLKRLE AEAIHIFRET VAETENPVML YSIGKDSSVL 70 80 90 100 110 120 LHLALKAFAP GRLPFPLLHV DTTWKFREMI AFRDARAKEL GLDLLVHTNP DGLARGVGPV 130 140 150 160 170 180 SHGSEVHTDV MKTQALRQAL DKHKFDAAFG GARRDEEASR AKERIISLRT AQHRWDPKRQ 190 200 210 220 230 240 RAEPWHLYNL KKKRGESLRV FPLSNWTELD IWLYIEQENI PIVPLYFAQE RPVVEREGQL 250 260 270 280 290 300 IMVDDERLPL EPGETPQQRL VRFRTLGCYP LTGAVESDAA TLPEIIGETL AARTSERQGR 310 VIDKDGAGAM ERKKQEGYF

References

[1]

"Complete sequence of chromosome of Methylobacterium radiotolerans JCM 2831." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C.J., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27329 / DSM 1819 / JCM 2831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001001 Genomic DNA. Translation: ACB23782.1.
RefSeq	YP_001754465.1. NC_010505.1.
3D structure databases
ProteinModelPortal	B1LSA1.
SMR	B1LSA1. Positions 14-228.
ModBase	Search...
Protein-protein interaction databases
STRING	B1LSA1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6137816.
GenomeReviews	Gene locus Mrad2831_1787 in contig CP001001_GR.
KEGG	mrd:Mrad2831_1787.
PATRIC	22571880. VBIMetRad70578_1838.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG758022.
OMA	NARIHPG.
ProtClustDB	PRK05253.
Family and domain databases
HAMAP	MF_00064. Sulf_adenylyltr_sub2. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K00957.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_METRJ

Accession

Primary (citable) accession number: B1LSA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program