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B1LR24 (B1LR24_ECOSM) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Thymidylate synthase HAMAP MF_00008
Short name=TS HAMAP MF_00008
Short name=TSase HAMAP MF_00008
EC=2.1.1.45 HAMAP MF_00008
Gene names
Name:thyA HAMAP MF_00008
Ordered Locus Names:EcSMS35_2974
OrganismEscherichia coli (strain SMS-3-5 / SECEC) [Complete proteome] [HAMAP] EMBL ACB20158.1
Taxonomic identifier439855 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008 SAAS SAAS023451

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008 SAAS SAAS023451

Subunit structure

Homodimer By similarity. HAMAP MF_00008 SAAS SAAS023451

Subcellular location

Cytoplasm By similarity HAMAP MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily. HAMAP MF_00008

Ontologies

Keywords
   Biological processNucleotide biosynthesis HAMAP MF_00008 SAAS SAAS023451
   Cellular componentCytoplasm HAMAP MF_00008
   Molecular functionMethyltransferase HAMAP MF_00008 SAAS SAAS023451 EMBL ACB20158.1
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdTMP biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionthymidylate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1461 By similarity HAMAP MF_00008

Sequences

Sequence LengthMass (Da)Tools
B1LR24 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 0E6D88ED98D24D22

FASTA26430,480
        10         20         30         40         50         60 
MKQYLELMQK VLDEGTQKND RTGTGTLSIF GHQMRFNLQD GFPLVTTKRC HLRSIIHELL 

        70         80         90        100        110        120 
WFLQGDTNIA YLHENNVTIW DEWADENGDL GPVYGKQWRA WPTPDGRHID QITTVLNQLK 

       130        140        150        160        170        180 
NDPDSRRIIV SAWNVGELDK MALAPCHAFF QFYVADGKLS CQLYQRSCDV FLGLPFNIAS 

       190        200        210        220        230        240 
YALLVHMMAQ QCDLEVGDFV WTGGDTHLYS NHMDQTHLQL SREPRPLPKL IIKRKPESIF 

       250        260 
DYRFEDFEIE GYDPHPGIKA PVAI

« Hide

References

[1]"Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5."
Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R.
J. Bacteriol. 190:6779-6794(2008) [PubMed: 18708504] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000970 Genomic DNA. Translation: ACB20158.1.
RefSeqYP_001744995.1. NC_010498.1.

3D structure databases

ProteinModelPortalB1LR24.
SMRB1LR24. Positions 2-264.
ModBaseSearch...

Protein-protein interaction databases

STRINGB1LR24.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000064392; EBESCP00000062034; EBESCG00000063439.
GeneID6144583.
GenomeReviewsGene locus EcSMS35_2974 in contig CP000970_GR.
KEGGecm:EcSMS35_2974.
PATRIC18434862. VBIEscCol6161_3127.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000009308.
HOGENOMHBG588098.
OMADIPRMKL.
ProtClustDBPRK01827.

Family and domain databases

HAMAPMF_00008. Thymidy_synth_bact.
[Tree]
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
Gene3DG3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KOK00560.
PANTHERPTHR11549:SF2. Thymidylat_synth_C. 1 hit.
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMsTIGR03284. Thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB1LR24_ECOSM
AccessionPrimary (citable) accession number: B1LR24
Entry history
Integrated into UniProtKB/TrEMBL: April 29, 2008
Last sequence update: April 29, 2008
Last modified: January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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