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B1LQY7

- QUEF_ECOSM

UniProt

B1LQY7 - QUEF_ECOSM

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Protein
NADPH-dependent 7-cyano-7-deazaguanine reductase
Gene
queF, EcSMS35_2934
Organism
Escherichia coli (strain SMS-3-5 / SECEC)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.UniRule annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei190 – 1901Thioimide intermediate By similarity
Active sitei197 – 1971Proton donor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi90 – 912NADPH By similarity
Nucleotide bindingi258 – 2592NADPH By similarity

GO - Molecular functioni

  1. oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Source: InterPro
  2. preQ1 synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciECOL439855:GHHB-2930-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase (EC:1.7.1.13)
Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase
Gene namesi
Name:queF
Ordered Locus Names:EcSMS35_2934
OrganismiEscherichia coli (strain SMS-3-5 / SECEC)
Taxonomic identifieri439855 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000007011: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation
PRO_1000213066Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi439855.EcSMS35_2934.

Structurei

3D structure databases

ProteinModelPortaliB1LQY7.
SMRiB1LQY7. Positions 21-282.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni88 – 903Substrate binding By similarity
Regioni229 – 2302Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0780.
HOGENOMiHOG000273755.
KOiK06879.
OMAiIFCDLMH.
OrthoDBiEOG6D5G0M.

Family and domain databases

HAMAPiMF_00817. QueF_type2.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.

Sequencei

Sequence statusi: Complete.

B1LQY7-1 [UniParc]FASTAAdd to Basket

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MSSYANHQAL AGLTLGKSTD YRDTYDASLL QGVPRSLNRD PLGLKADNLP    50
FHGTDIWTLY ELSWLNAKGL PQVAVGHVEL DYTSANLIES KSFKLYLNSF 100
NQTRFNNWDE VRQTLERDLS TCAQGKVSVA LYRLDELEGQ PIGHFNGTCI 150
DDQDITIDNY EFTTDYLENA TSGEKVVEET LVSHLLKSNC LITHQPDWGS 200
IQIQYRGRQI DREKLLRYLV SFRHHNEFHE QCVERIFNDL LRFCQPEKLS 250
VYARYTRRGG LDINPWRSNS DFVPSTTRLV RQ 282
Length:282
Mass (Da):32,529
Last modified:April 29, 2008 - v1
Checksum:i83B7798A769831DE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000970 Genomic DNA. Translation: ACB19943.1.
RefSeqiYP_001744958.1. NC_010498.1.

Genome annotation databases

EnsemblBacteriaiACB19943; ACB19943; EcSMS35_2934.
GeneIDi6142799.
KEGGiecm:EcSMS35_2934.
PATRICi18434782. VBIEscCol6161_3090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000970 Genomic DNA. Translation: ACB19943.1 .
RefSeqi YP_001744958.1. NC_010498.1.

3D structure databases

ProteinModelPortali B1LQY7.
SMRi B1LQY7. Positions 21-282.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 439855.EcSMS35_2934.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB19943 ; ACB19943 ; EcSMS35_2934 .
GeneIDi 6142799.
KEGGi ecm:EcSMS35_2934.
PATRICi 18434782. VBIEscCol6161_3090.

Phylogenomic databases

eggNOGi COG0780.
HOGENOMi HOG000273755.
KOi K06879.
OMAi IFCDLMH.
OrthoDBi EOG6D5G0M.

Enzyme and pathway databases

UniPathwayi UPA00392 .
BioCyci ECOL439855:GHHB-2930-MONOMER.

Family and domain databases

HAMAPi MF_00817. QueF_type2.
InterProi IPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view ]
Pfami PF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsi TIGR03138. QueF. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5."
    Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R.
    J. Bacteriol. 190:6779-6794(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SMS-3-5 / SECEC.

Entry informationi

Entry nameiQUEF_ECOSM
AccessioniPrimary (citable) accession number: B1LQY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: April 29, 2008
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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