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B1LQY7

- QUEF_ECOSM

UniProt

B1LQY7 - QUEF_ECOSM

Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Escherichia coli (strain SMS-3-5 / SECEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

    Catalytic activityi

    7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei190 – 1901Thioimide intermediateUniRule annotation
    Active sitei197 – 1971Proton donorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi90 – 912NADPHUniRule annotation
    Nucleotide bindingi258 – 2592NADPHUniRule annotation

    GO - Molecular functioni

    1. oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Source: InterPro
    2. preQ1 synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. queuosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Queuosine biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciECOL439855:GHHB-2930-MONOMER.
    UniPathwayiUPA00392.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
    Alternative name(s):
    7-cyano-7-carbaguanine reductaseUniRule annotation
    NADPH-dependent nitrile oxidoreductaseUniRule annotation
    PreQ(0) reductaseUniRule annotation
    Gene namesi
    Name:queFUniRule annotation
    Ordered Locus Names:EcSMS35_2934
    OrganismiEscherichia coli (strain SMS-3-5 / SECEC)
    Taxonomic identifieri439855 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000007011: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 282282NADPH-dependent 7-cyano-7-deazaguanine reductasePRO_1000213066Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi439855.EcSMS35_2934.

    Structurei

    3D structure databases

    ProteinModelPortaliB1LQY7.
    SMRiB1LQY7. Positions 21-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni88 – 903Substrate bindingUniRule annotation
    Regioni229 – 2302Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0780.
    HOGENOMiHOG000273755.
    KOiK06879.
    OMAiIFCDLMH.
    OrthoDBiEOG6D5G0M.

    Family and domain databases

    HAMAPiMF_00817. QueF_type2.
    InterProiIPR029500. QueF.
    IPR029139. QueF_N.
    IPR016428. QueF_type2.
    [Graphical view]
    PfamiPF14489. QueF. 1 hit.
    PF14819. QueF_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
    TIGRFAMsiTIGR03138. QueF. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B1LQY7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSYANHQAL AGLTLGKSTD YRDTYDASLL QGVPRSLNRD PLGLKADNLP    50
    FHGTDIWTLY ELSWLNAKGL PQVAVGHVEL DYTSANLIES KSFKLYLNSF 100
    NQTRFNNWDE VRQTLERDLS TCAQGKVSVA LYRLDELEGQ PIGHFNGTCI 150
    DDQDITIDNY EFTTDYLENA TSGEKVVEET LVSHLLKSNC LITHQPDWGS 200
    IQIQYRGRQI DREKLLRYLV SFRHHNEFHE QCVERIFNDL LRFCQPEKLS 250
    VYARYTRRGG LDINPWRSNS DFVPSTTRLV RQ 282
    Length:282
    Mass (Da):32,529
    Last modified:April 29, 2008 - v1
    Checksum:i83B7798A769831DE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000970 Genomic DNA. Translation: ACB19943.1.
    RefSeqiYP_001744958.1. NC_010498.1.

    Genome annotation databases

    EnsemblBacteriaiACB19943; ACB19943; EcSMS35_2934.
    GeneIDi6142799.
    KEGGiecm:EcSMS35_2934.
    PATRICi18434782. VBIEscCol6161_3090.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000970 Genomic DNA. Translation: ACB19943.1 .
    RefSeqi YP_001744958.1. NC_010498.1.

    3D structure databases

    ProteinModelPortali B1LQY7.
    SMRi B1LQY7. Positions 21-282.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 439855.EcSMS35_2934.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB19943 ; ACB19943 ; EcSMS35_2934 .
    GeneIDi 6142799.
    KEGGi ecm:EcSMS35_2934.
    PATRICi 18434782. VBIEscCol6161_3090.

    Phylogenomic databases

    eggNOGi COG0780.
    HOGENOMi HOG000273755.
    KOi K06879.
    OMAi IFCDLMH.
    OrthoDBi EOG6D5G0M.

    Enzyme and pathway databases

    UniPathwayi UPA00392 .
    BioCyci ECOL439855:GHHB-2930-MONOMER.

    Family and domain databases

    HAMAPi MF_00817. QueF_type2.
    InterProi IPR029500. QueF.
    IPR029139. QueF_N.
    IPR016428. QueF_type2.
    [Graphical view ]
    Pfami PF14489. QueF. 1 hit.
    PF14819. QueF_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
    TIGRFAMsi TIGR03138. QueF. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5."
      Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R.
      J. Bacteriol. 190:6779-6794(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SMS-3-5 / SECEC.

    Entry informationi

    Entry nameiQUEF_ECOSM
    AccessioniPrimary (citable) accession number: B1LQY7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3