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Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Escherichia coli (strain SMS-3-5 / SECEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei190Thioimide intermediateUniRule annotation1
Active sitei197Proton donorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi90 – 91NADPHUniRule annotation2
Nucleotide bindingi258 – 259NADPHUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:EcSMS35_2934
OrganismiEscherichia coli (strain SMS-3-5 / SECEC)
Taxonomic identifieri439855 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000007011 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002130661 – 282NADPH-dependent 7-cyano-7-deazaguanine reductaseAdd BLAST282

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1LQY7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni88 – 90Substrate bindingUniRule annotation3
Regioni229 – 230Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000273755.
KOiK06879.
OMAiQCVERIY.

Family and domain databases

HAMAPiMF_00817. QueF_type2. 1 hit.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.

Sequencei

Sequence statusi: Complete.

B1LQY7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSYANHQAL AGLTLGKSTD YRDTYDASLL QGVPRSLNRD PLGLKADNLP
60 70 80 90 100
FHGTDIWTLY ELSWLNAKGL PQVAVGHVEL DYTSANLIES KSFKLYLNSF
110 120 130 140 150
NQTRFNNWDE VRQTLERDLS TCAQGKVSVA LYRLDELEGQ PIGHFNGTCI
160 170 180 190 200
DDQDITIDNY EFTTDYLENA TSGEKVVEET LVSHLLKSNC LITHQPDWGS
210 220 230 240 250
IQIQYRGRQI DREKLLRYLV SFRHHNEFHE QCVERIFNDL LRFCQPEKLS
260 270 280
VYARYTRRGG LDINPWRSNS DFVPSTTRLV RQ
Length:282
Mass (Da):32,529
Last modified:April 29, 2008 - v1
Checksum:i83B7798A769831DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000970 Genomic DNA. Translation: ACB19943.1.
RefSeqiWP_000100411.1. NC_010498.1.

Genome annotation databases

EnsemblBacteriaiACB19943; ACB19943; EcSMS35_2934.
KEGGiecm:EcSMS35_2934.
PATRICi18434782. VBIEscCol6161_3090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000970 Genomic DNA. Translation: ACB19943.1.
RefSeqiWP_000100411.1. NC_010498.1.

3D structure databases

ProteinModelPortaliB1LQY7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB19943; ACB19943; EcSMS35_2934.
KEGGiecm:EcSMS35_2934.
PATRICi18434782. VBIEscCol6161_3090.

Phylogenomic databases

HOGENOMiHOG000273755.
KOiK06879.
OMAiQCVERIY.

Enzyme and pathway databases

UniPathwayiUPA00392.

Family and domain databases

HAMAPiMF_00817. QueF_type2. 1 hit.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQUEF_ECOSM
AccessioniPrimary (citable) accession number: B1LQY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.