B1LPK6 (PLSB_ECOSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	EcSMS35_4503

Organism

Escherichia coli (strain SMS-3-5 / SECEC) [Complete proteome] [HAMAP]

Taxonomic identifier

439855 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 827	827	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000123082
Regions
	Motif	325 – 330	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	B1LPK6 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: AFAE24FC9859B824	FASTA	827	93,724
	10 20 30 40 50 60 MTFCYPCRAF ALLTRGFTSF MSGWPRIYYK LLNLPLSILV KSKSIPADPA PELGLDTSRP 70 80 90 100 110 120 IMYVLPYNSK ADLLTLRAQC LAHDLPDPLE PLEIDGTLLP RYVFIHGGPR VFTYYTPKEE 130 140 150 160 170 180 SIKLFHDYLD LHRSNPNLDV QMVPVSVMFG RAPGREKGEV NPPLRMLNGV QKFFAVLWLG 190 200 210 220 230 240 RDSFVRFSPS VSLRRMADEH GTDKTIAQKL ARVARMHFAR QRLAAVGPRL PARQDLFNKL 250 260 270 280 290 300 LASRAIAKAV EDEARSKKIS HEKAQQNAIV LMEEIAANFS YEMIRLTDRI LGFTWNRLYQ 310 320 330 340 350 360 GINVHNAERV RQLAHDGHEL VYVPCHRSHM DYLLLSYVLY HQGLVPPHIA AGINLNFWPA 370 380 390 400 410 420 GPIFRRLGAF FIRRTFKGNK LYSTVFREYL GELFSRGYSV EYFVEGGRSR TGRLLDPKTG 430 440 450 460 470 480 TLSMTIQAML RGGTRPITLI PIYIGYEHVM EVGTYAKELR GATKEKESLP QMLRGLSKLR 490 500 510 520 530 540 NLGQGYVNFG EPMPLMTYLN QHVPDWRESI DPIEAVRPAW LTPTVNNIAA DLMVRINNAG 550 560 570 580 590 600 AANAMNLCCT ALLASRQRSL TREQLTEQLN CYLDLMRNVP YSTDSTVPSA SASELIDHAL 610 620 630 640 650 660 QMNKFEVEKD TIGDIIILPR EQAVLMTYYR NNIAHMLVLP SLMAAIVTQH RHISRDVLME 670 680 690 700 710 720 HVNVLYPMLK AELFLRWDRD ELPDVIDALA NEMQRQGLIT LQDDELHINP AHSRTLQLLA 730 740 750 760 770 780 AGARETLQRY AITFWLLSAN PSINRGTLEK ESRTVAQRLS VLHGINAPEF FDKAVFSSLV 790 800 810 820 LTLRDEGYIS DSGDAEPAET MKVYQLLAEL ITSDVRLTIE SATQGEG

References

[1]

"Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5." Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R. J. Bacteriol. 190:6779-6794(2008) [PubMed: 18708504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SMS-3-5 / SECEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000970 Genomic DNA. Translation: ACB18864.1.
RefSeq	YP_001746431.1. NC_010498.1.
3D structure databases
ProteinModelPortal	B1LPK6.
ModBase	Search...
Protein-protein interaction databases
STRING	B1LPK6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000061492; EBESCP00000059134; EBESCG00000060539.
GeneID	6145679.
GenomeReviews	Gene locus EcSMS35_4503 in contig CP000970_GR.
KEGG	ecm:EcSMS35_4503.
PATRIC	18437926. VBIEscCol6161_4618.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010697.
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Enzyme and pathway databases
BioCyc	ECOL439855:ECSMS35_4503-MONOMER.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_ECOSM

Accession

Primary (citable) accession number: B1LPK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program