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Protein

1,4-dihydroxy-2-naphthoate octaprenyltransferase

Gene

menA

Organism
Escherichia coli (strain SMS-3-5 / SECEC)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Conversion of 1,4-dihydroxy-2-naphthoate (DHNA) to demethylmenaquinone (DMK).UniRule annotation

Catalytic activityi

An all-trans-polyprenyl diphosphate + 1,4-dihydroxy-2-naphthoate = a demethylmenaquinol + diphosphate + CO2.UniRule annotation

Pathway: menaquinone biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1,4-dihydroxy-2-naphthoate octaprenyltransferase (menA)
  2. Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE (ubiE)
This subpathway is part of the pathway menaquinone biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate, the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationImported

Keywords - Biological processi

Menaquinone biosynthesisUniRule annotation

Enzyme and pathway databases

BioCyciECOL439855:GHHB-4366-MONOMER.
UniPathwayiUPA00079; UER00168.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-dihydroxy-2-naphthoate octaprenyltransferaseUniRule annotation (EC:2.5.1.74UniRule annotation)
Short name:
DHNA-octaprenyltransferaseUniRule annotation
Gene namesi
Name:menAUniRule annotationImported
Ordered Locus Names:EcSMS35_4372Imported
OrganismiEscherichia coli (strain SMS-3-5 / SECEC)Imported
Taxonomic identifieri439855 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000007011 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei25 – 4521HelicalUniRule annotationAdd
BLAST
Transmembranei47 – 6721HelicalUniRule annotationAdd
BLAST
Transmembranei102 – 12221HelicalUniRule annotationAdd
BLAST
Transmembranei128 – 14821HelicalUniRule annotationAdd
BLAST
Transmembranei153 – 17321HelicalUniRule annotationAdd
BLAST
Transmembranei181 – 20121HelicalUniRule annotationAdd
BLAST
Transmembranei232 – 25120HelicalUniRule annotationAdd
BLAST
Transmembranei255 – 27420HelicalUniRule annotationAdd
BLAST
Transmembranei291 – 31121HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membraneUniRule annotation, Cell membrane, Membrane

Family & Domainsi

Sequence similaritiesi

Belongs to the MenA family. Type 1 subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixUniRule annotation

Phylogenomic databases

eggNOGiCOG1575.
HOGENOMiHOG000028297.
KOiK02548.
OMAiYTGGKKP.
OrthoDBiEOG6W7220.

Family and domain databases

HAMAPiMF_01937. MenA_1.
InterProiIPR004657. MenA.
IPR000537. UbiA_prenyltransferase.
IPR026046. UBIAD1.
[Graphical view]
PfamiPF01040. UbiA. 1 hit.
[Graphical view]
PIRSFiPIRSF005355. UBIAD1. 1 hit.

Sequencei

Sequence statusi: Complete.

B1LNN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARIMTEQQI SRTQAWLESL RPKTLPLAFA AIIVGTALAW WQGHFDPLVA
60 70 80 90 100
LLALITAGLL QILSNLANDY GDAVKGSDKP DRIGPLRGMQ KGVITQQEMK
110 120 130 140 150
RALIITVVLI CLSGLALVAV ACHTLADFVG FLILGGLSII AAITYTVGNR
160 170 180 190 200
PYGYIGLGDI SVLVFFGWLS VMGSWYLQAH TLIPALILPA TACGLLATAV
210 220 230 240 250
LNINNLRDIN SDRENGKNTL VVRLGAVNAR RYHACLLMGS LVCLALFNLF
260 270 280 290 300
SLHSLWGWLF LLAAPLLVKQ ARYVMREMDP VAMRPMLERT VKGALLTNLL
310
FVLGIFLSQW AA
Length:312
Mass (Da):34,008
Last modified:April 29, 2008 - v1
Checksum:i4F381C518D026A55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000970 Genomic DNA. Translation: ACB18241.1.
RefSeqiWP_000101132.1. NC_010498.1.
YP_001746317.1. NC_010498.1.

Genome annotation databases

EnsemblBacteriaiACB18241; ACB18241; EcSMS35_4372.
KEGGiecm:EcSMS35_4372.
PATRICi18437664. VBIEscCol6161_4499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000970 Genomic DNA. Translation: ACB18241.1.
RefSeqiWP_000101132.1. NC_010498.1.
YP_001746317.1. NC_010498.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB18241; ACB18241; EcSMS35_4372.
KEGGiecm:EcSMS35_4372.
PATRICi18437664. VBIEscCol6161_4499.

Phylogenomic databases

eggNOGiCOG1575.
HOGENOMiHOG000028297.
KOiK02548.
OMAiYTGGKKP.
OrthoDBiEOG6W7220.

Enzyme and pathway databases

UniPathwayiUPA00079; UER00168.
BioCyciECOL439855:GHHB-4366-MONOMER.

Family and domain databases

HAMAPiMF_01937. MenA_1.
InterProiIPR004657. MenA.
IPR000537. UbiA_prenyltransferase.
IPR026046. UBIAD1.
[Graphical view]
PfamiPF01040. UbiA. 1 hit.
[Graphical view]
PIRSFiPIRSF005355. UBIAD1. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5."
    Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R.
    J. Bacteriol. 190:6779-6794(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SMS-3-5 / SECECImported.

Entry informationi

Entry nameiB1LNN5_ECOSM
AccessioniPrimary (citable) accession number: B1LNN5
Entry historyi
Integrated into UniProtKB/TrEMBL: April 29, 2008
Last sequence update: April 29, 2008
Last modified: June 24, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.