B1LMN0 (HEM6_ECOSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coproporphyrinogen-III oxidase, aerobic
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	EcSMS35_2591

Organism

Escherichia coli (strain SMS-3-5 / SECEC) [Complete proteome] [HAMAP]

Taxonomic identifier

439855 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Cofactor	Manganese By similarity. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Manganese
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_1000119802
Regions
	Region	48 – 57	10	Important for dimerization By similarity
	Region	108 – 110	3	Substrate binding By similarity
	Region	240 – 275	36	Important for dimerization By similarity
	Region	258 – 263	6	Substrate binding By similarity
Sites
	Active site	106	1	Proton donor By similarity
	Binding site	92	1	Substrate By similarity
	Site	175	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1LMN0 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 0AB6A5B9B381E566	FASTA	299	34,388
	10 20 30 40 50 60 MKPDAHQVKQ FLLNLQDTIC QQLSAVDGAE FVEDSWLREA GGGGRSRVLR NGGVFEQAGV 70 80 90 100 110 120 NFSHVHGEVM PASATAHRPE LAGRSFEAMG VSLVVHPHNP YVPTSHANVR FFIAEKPGAE 130 140 150 160 170 180 PVWWFGGGFD LTPFYGFEED AIHWHRTARD LCQPFGEDVY PRYKKWCDEY FYLKHRNEQR 190 200 210 220 230 240 GIGGLFFDDL NTPDFDHCFA FMQAVGKGYT NAYLPIVERR KTMAYGERER NFQLYRRGRY 250 260 270 280 290 VEFNLVWDRG TLFGLQTGGR TESILMSMPP LVRWEYDYQP KDGSPEAALN EFIKVRDWV

References

[1]

"Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5." Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R. J. Bacteriol. 190:6779-6794(2008) [PubMed: 18708504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SMS-3-5 / SECEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000970 Genomic DNA. Translation: ACB15546.1.
RefSeq	YP_001744627.1. NC_010498.1.
3D structure databases
ProteinModelPortal	B1LMN0.
SMR	B1LMN0. Positions 8-299.
ModBase	Search...
Protein-protein interaction databases
STRING	B1LMN0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000061313; EBESCP00000058955; EBESCG00000060360.
GeneID	6142944.
GenomeReviews	Gene locus EcSMS35_2591 in contig CP000970_GR.
KEGG	ecm:EcSMS35_2591.
PATRIC	18434080. VBIEscCol6161_2749.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010136.
HOGENOM	HBG631180.
OMA	VKAYLLD.
ProtClustDB	PRK05330.
Enzyme and pathway databases
BioCyc	ECOL439855:ECSMS35_2591-MONOMER.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidase_aer. IPR018375. Coprogen_oxidase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
KO	K00228.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
SUPFAM	SSF102886. Coprogen_oxidas. 1 hit.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_ECOSM

Accession

Primary (citable) accession number: B1LMN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program