B1LLG1 (SUCC_ECOSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinyl-CoA ligase [ADP-forming] subunit beta
EC=6.2.1.5

Alternative name(s):
Succinyl-CoA synthetase subunit beta
Short name=SCS-beta

Gene names

Name:	sucC
Ordered Locus Names:	EcSMS35_0740

Organism

Escherichia coli (strain SMS-3-5 / SECEC) [Complete proteome] [HAMAP]

Taxonomic identifier

439855 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + succinate + CoA = ADP + phosphate + succinyl-CoA. HAMAP-Rule MF_00558
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1. HAMAP-Rule MF_00558
Subunit structure	Heterotetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the succinate/malate CoA ligase beta subunit family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP succinate-CoA ligase (ADP-forming) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Succinyl-CoA ligase [ADP-forming] subunit beta HAMAP-Rule MF_00558		PRO_1000129188
Regions
	Domain	9 – 244	236	ATP-grasp
	Nucleotide binding	35 – 108	74	ATP By similarity
Sites
	Metal binding	197	1	Magnesium or manganese By similarity
	Metal binding	199	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1LLG1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 09C429EC97A823CF	FASTA	388	41,393
	10 20 30 40 50 60 MNLHEYQAKQ LFARYGLPAP VGYACTTPRE AEEAASKIGA GPWVVKCQVH AGGRGKAGGV 70 80 90 100 110 120 KVVNSKEDIR AFAENWLGKR LVTYQTDANG QPVNQILVEA ATDIAKELYL GAVVDRSSRR 130 140 150 160 170 180 VVFMASTEGG VEIEKVAEET PHLIHKVALD PLTGPMPYQG RELAFKLGLE GKLVQQFTKI 190 200 210 220 230 240 FMGLATIFLE RDLALIEINP LVITKQGDLI CLDGKLGADG NALFRQPDLR EMRDQSQEDP 250 260 270 280 290 300 REAQAAQWEL NYVALDGNIG CMVNGAGLAM GTMDIVKLHG GEPANFLDVG GGATKERVTE 310 320 330 340 350 360 AFKIILSDDK VKAVLVNIFG GIVRCDLIAD GIIGAVAEVG VNVPVVVRLE GNNAELGAKK 370 380 LADSGLNIIA AKGLTDAAQQ VVAAVEGK

References

[1]

"Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5." Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R. J. Bacteriol. 190:6779-6794(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SMS-3-5 / SECEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000970 Genomic DNA. Translation: ACB19410.1.
RefSeq	YP_001742828.1. NC_010498.1.
3D structure databases
ProteinModelPortal	B1LLG1.
SMR	B1LLG1. Positions 1-388.
ModBase	Search...
Protein-protein interaction databases
STRING	439855.EcSMS35_0740.
Proteomic databases
PRIDE	B1LLG1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB19410; ACB19410; EcSMS35_0740.
GeneID	6145198.
KEGG	ecm:EcSMS35_0740.
PATRIC	18430317. VBIEscCol6161_0902.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0045.
HOGENOM	HOG000007059.
KO	K01903.
OMA	QPVTKIL.
ProtClustDB	PRK00696.
Enzyme and pathway databases
BioCyc	ECOL439855:GHHB-4014-MONOMER.
UniPathway	UPA00223; UER00999.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.261. 1 hit.
HAMAP	MF_00558. Succ_CoA_beta.
InterPro	IPR011761. ATP-grasp. IPR013650. ATP-grasp_succ-CoA_synth-type. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR005811. CoA_ligase. IPR017866. Succ-CoA_synthase_bsu_CS. IPR005809. Succ_CoA_synthase_bsu. IPR016102. Succinyl-CoA_synth-like. [Graphical view]
PANTHER	PTHR11815. PTHR11815. 1 hit.
Pfam	PF08442. ATP-grasp_2. 1 hit. PF00549. Ligase_CoA. 1 hit. [Graphical view]
PIRSF	PIRSF001554. SucCS_beta. 1 hit.
SUPFAM	SSF52210. CoA_ligase. 1 hit.
TIGRFAMs	TIGR01016. sucCoAbeta. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS01217. SUCCINYL_COA_LIG_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SUCC_ECOSM

Accession

Primary (citable) accession number: B1LLG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program