Reviewed, UniProtKB/Swiss-Prot B1LJS7 (SSUD_ECOSM)

Names and origin

Protein names

Recommended name:
    Alkanesulfonate monooxygenase
    EC=1.14.14.5
Alternative name(s):
    FMNH2-dependent aliphatic sulfonate monooxygenase

Gene names

Name:	ssuD
Ordered Locus Names:	EcSMS35_2184

Organism

Escherichia coli (strain SMS-3-5 / SECEC) [Complete proteome] [HAMAP]

Taxonomic identifier

439855 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the desulfonation of aliphatic sulfonates By similarity.
Catalytic activity	An alkanesufonate (R-CH(2)-SO3H) + FMNH2 + O2 = an aldehyde (R-CHO) + FMN + sulfite + H2O. HAMAP MF_01229
Subunit structure	Homotetramer By similarity.
Miscellaneous	FMNH2 which is absolutely required for this enzymatic reaction, is provided by ssuE By similarity.
Sequence similarities	Belongs to the ssuD family.

Ontologies

Keywords
Ligand	FMN
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanesulfonate monooxygenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Alkanesulfonate monooxygenase HAMAP MF_01229		PRO_1000139623

Sequences

	Sequence		Length	Mass (Da)
	B1LJS7-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 74DF92DBB7D4D7A8	FASTA	381	41,600
	10 20 30 40 50 60 MSLNMFWFLP THGDGHYLGT EEGSRPVDHG YLQQIAQAAD RLGYTGVLIP TGRSCEDAWL 70 80 90 100 110 120 VAASMIPVTQ RLKFLVALRP SVTSPTVAAR QAATLDRLSN GRALFNLVTG SDPQELAGDG 130 140 150 160 170 180 VFLDHSERYE ASAEFTQVWR RLLLGETVDF NGKHIHVRGA KLLFPPIQQP YPPLYFGGSS 190 200 210 220 230 240 DVAQELAAEQ VDLYLTWGEP PELVKEKIEQ VRAKAAAHGR KIRFGIRLHV IVRETNGEAW 250 260 270 280 290 300 QAAERLISHL DDETIAKAQA AFARTDSVGQ QRMAALHNGK RDNLEISPNL WAGVGLVRGG 310 320 330 340 350 360 AGTALVGDGP TVAARINEYA ALGIDSFVLS GYPHLEEAYR VGELLFPHLD VAIPEIPQPQ 370 380 PLNPQGEAVA NDFIPRNVAQ S

References

[1]

"Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5." Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R. J. Bacteriol. 190:6779-6794(2008) [PubMed: 18708504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000970 Genomic DNA. Translation: ACB16159.1.
RefSeq	YP_001744234.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6142745.
GenomeReviews	Gene locus EcSMS35_2184 in contig CP000970_GR.
KEGG	ecm:EcSMS35_2184.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NIFWFLP.
Family and domain databases
HAMAP	MF_01229. [Tree]
InterPro	IPR019911. Alkanesulphonate_mOase_FMN-dep. IPR011251. Luciferase-like. IPR016048. Luciferase-like_sub. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03565. Alk_sulf_monoox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SSUD_ECOSM

Accession

Primary (citable) accession number: B1LJS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)