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B1LJF4

- TGT_ECOSM

UniProt

B1LJF4 - TGT_ECOSM

Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Escherichia coli (strain SMS-3-5 / SECEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

    Catalytic activityi

    Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
    Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891NucleophileUniRule annotation
    Binding sitei90 – 901SubstrateUniRule annotation
    Metal bindingi302 – 3021ZincUniRule annotation
    Metal bindingi304 – 3041ZincUniRule annotation
    Metal bindingi307 – 3071ZincUniRule annotation
    Metal bindingi333 – 3331ZincUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. queuosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Queuosine biosynthesis, tRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciECOL439855:GHHB-436-MONOMER.
    UniPathwayiUPA00392.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
    Alternative name(s):
    Guanine insertion enzymeUniRule annotation
    tRNA-guanine transglycosylaseUniRule annotation
    Gene namesi
    Name:tgtUniRule annotation
    Ordered Locus Names:EcSMS35_0438
    OrganismiEscherichia coli (strain SMS-3-5 / SECEC)
    Taxonomic identifieri439855 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000007011: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 375375Queuine tRNA-ribosyltransferasePRO_1000198001Add
    BLAST

    Proteomic databases

    PRIDEiB1LJF4.

    Interactioni

    Protein-protein interaction databases

    STRINGi439855.EcSMS35_0438.

    Structurei

    3D structure databases

    ProteinModelPortaliB1LJF4.
    SMRiB1LJF4. Positions 3-364.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0343.
    HOGENOMiHOG000223473.
    KOiK00773.
    OMAiFGSDIIM.
    OrthoDBiEOG6SNDVG.

    Family and domain databases

    Gene3Di3.20.20.105. 1 hit.
    HAMAPiMF_00168. Q_tRNA_Tgt.
    InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
    IPR002616. tRNA_ribo_trans-like.
    [Graphical view]
    PANTHERiPTHR11962. PTHR11962. 1 hit.
    PfamiPF01702. TGT. 1 hit.
    [Graphical view]
    SUPFAMiSSF51713. SSF51713. 1 hit.
    TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
    TIGR00449. tgt_general. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B1LJF4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFELDTTDG RARRGRLVFD RGVVETPCFM PVGTYGTVKG MTPEEVEATG    50
    AQIILGNTFH LWLRPGQEIM KLHGDLHDFM QWKGPILTDS GGFQVFSLGD 100
    IRKITEQGVH FRNPINGDPI FLDPEKSMEI QYDLGSDIVM IFDECTPYPA 150
    DWDYAKRSME MSLRWAKRSR ERFDSLGNKN ALFGIIQGSV YEDLRDISVK 200
    GLVDIGFDGY AVGGLAVGEP KADMHRILEH VCPQIPADKP RYLMGVGKPE 250
    DLVEGVRRGI DMFDCVMPTR NARNGHLFVT DGVVKIRNAK YKSDTGPLDP 300
    ECDCYTCRNY SRAYLHHLDR CNEILGARLN TIHNLRYYQR LMAGLRKAIE 350
    EGKLESFVTD FYQRQGREVP PLNVD 375
    Length:375
    Mass (Da):42,594
    Last modified:April 29, 2008 - v1
    Checksum:i40271CA08D8A8820
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000970 Genomic DNA. Translation: ACB16174.1.
    RefSeqiYP_001742541.1. NC_010498.1.

    Genome annotation databases

    EnsemblBacteriaiACB16174; ACB16174; EcSMS35_0438.
    GeneIDi6146420.
    KEGGiecm:EcSMS35_0438.
    PATRICi18429719. VBIEscCol6161_0611.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000970 Genomic DNA. Translation: ACB16174.1 .
    RefSeqi YP_001742541.1. NC_010498.1.

    3D structure databases

    ProteinModelPortali B1LJF4.
    SMRi B1LJF4. Positions 3-364.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 439855.EcSMS35_0438.

    Proteomic databases

    PRIDEi B1LJF4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB16174 ; ACB16174 ; EcSMS35_0438 .
    GeneIDi 6146420.
    KEGGi ecm:EcSMS35_0438.
    PATRICi 18429719. VBIEscCol6161_0611.

    Phylogenomic databases

    eggNOGi COG0343.
    HOGENOMi HOG000223473.
    KOi K00773.
    OMAi FGSDIIM.
    OrthoDBi EOG6SNDVG.

    Enzyme and pathway databases

    UniPathwayi UPA00392 .
    BioCyci ECOL439855:GHHB-436-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.105. 1 hit.
    HAMAPi MF_00168. Q_tRNA_Tgt.
    InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
    IPR002616. tRNA_ribo_trans-like.
    [Graphical view ]
    PANTHERi PTHR11962. PTHR11962. 1 hit.
    Pfami PF01702. TGT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51713. SSF51713. 1 hit.
    TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
    TIGR00449. tgt_general. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5."
      Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R.
      J. Bacteriol. 190:6779-6794(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SMS-3-5 / SECEC.

    Entry informationi

    Entry nameiTGT_ECOSM
    AccessioniPrimary (citable) accession number: B1LJF4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3