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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Escherichia coli (strain SMS-3-5 / SECEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891NucleophileUniRule annotation
Binding sitei90 – 901SubstrateUniRule annotation
Metal bindingi302 – 3021ZincUniRule annotation
Metal bindingi304 – 3041ZincUniRule annotation
Metal bindingi307 – 3071ZincUniRule annotation
Metal bindingi333 – 3331ZincUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciECOL439855:GHHB-436-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:EcSMS35_0438
OrganismiEscherichia coli (strain SMS-3-5 / SECEC)
Taxonomic identifieri439855 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000007011 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Queuine tRNA-ribosyltransferasePRO_1000198001Add
BLAST

Proteomic databases

PRIDEiB1LJF4.

Interactioni

Protein-protein interaction databases

STRINGi439855.EcSMS35_0438.

Structurei

3D structure databases

ProteinModelPortaliB1LJF4.
SMRiB1LJF4. Positions 3-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0343.
HOGENOMiHOG000223473.
KOiK00773.
OMAiTPRFMPV.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

B1LJF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFELDTTDG RARRGRLVFD RGVVETPCFM PVGTYGTVKG MTPEEVEATG
60 70 80 90 100
AQIILGNTFH LWLRPGQEIM KLHGDLHDFM QWKGPILTDS GGFQVFSLGD
110 120 130 140 150
IRKITEQGVH FRNPINGDPI FLDPEKSMEI QYDLGSDIVM IFDECTPYPA
160 170 180 190 200
DWDYAKRSME MSLRWAKRSR ERFDSLGNKN ALFGIIQGSV YEDLRDISVK
210 220 230 240 250
GLVDIGFDGY AVGGLAVGEP KADMHRILEH VCPQIPADKP RYLMGVGKPE
260 270 280 290 300
DLVEGVRRGI DMFDCVMPTR NARNGHLFVT DGVVKIRNAK YKSDTGPLDP
310 320 330 340 350
ECDCYTCRNY SRAYLHHLDR CNEILGARLN TIHNLRYYQR LMAGLRKAIE
360 370
EGKLESFVTD FYQRQGREVP PLNVD
Length:375
Mass (Da):42,594
Last modified:April 29, 2008 - v1
Checksum:i40271CA08D8A8820
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000970 Genomic DNA. Translation: ACB16174.1.
RefSeqiYP_001742541.1. NC_010498.1.

Genome annotation databases

EnsemblBacteriaiACB16174; ACB16174; EcSMS35_0438.
KEGGiecm:EcSMS35_0438.
PATRICi18429719. VBIEscCol6161_0611.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000970 Genomic DNA. Translation: ACB16174.1.
RefSeqiYP_001742541.1. NC_010498.1.

3D structure databases

ProteinModelPortaliB1LJF4.
SMRiB1LJF4. Positions 3-364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi439855.EcSMS35_0438.

Proteomic databases

PRIDEiB1LJF4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB16174; ACB16174; EcSMS35_0438.
KEGGiecm:EcSMS35_0438.
PATRICi18429719. VBIEscCol6161_0611.

Phylogenomic databases

eggNOGiCOG0343.
HOGENOMiHOG000223473.
KOiK00773.
OMAiTPRFMPV.
OrthoDBiEOG6SNDVG.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciECOL439855:GHHB-436-MONOMER.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5."
    Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R.
    J. Bacteriol. 190:6779-6794(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SMS-3-5 / SECEC.

Entry informationi

Entry nameiTGT_ECOSM
AccessioniPrimary (citable) accession number: B1LJF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: April 1, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.