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B1LJF4

- TGT_ECOSM

UniProt

B1LJF4 - TGT_ECOSM

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Protein
Queuine tRNA-ribosyltransferase
Gene
tgt, EcSMS35_0438
Organism
Escherichia coli (strain SMS-3-5 / SECEC)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Nucleophile By similarity
Binding sitei90 – 901Substrate By similarity
Metal bindingi302 – 3021Zinc By similarity
Metal bindingi304 – 3041Zinc By similarity
Metal bindingi307 – 3071Zinc By similarity
Metal bindingi333 – 3331Zinc By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciECOL439855:GHHB-436-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferase (EC:2.4.2.29)
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene namesi
Name:tgt
Ordered Locus Names:EcSMS35_0438
OrganismiEscherichia coli (strain SMS-3-5 / SECEC)
Taxonomic identifieri439855 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000007011: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Queuine tRNA-ribosyltransferaseUniRule annotation
PRO_1000198001Add
BLAST

Proteomic databases

PRIDEiB1LJF4.

Interactioni

Protein-protein interaction databases

STRINGi439855.EcSMS35_0438.

Structurei

3D structure databases

ProteinModelPortaliB1LJF4.
SMRiB1LJF4. Positions 3-364.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0343.
HOGENOMiHOG000223473.
KOiK00773.
OMAiFGSDIIM.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

B1LJF4-1 [UniParc]FASTAAdd to Basket

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MKFELDTTDG RARRGRLVFD RGVVETPCFM PVGTYGTVKG MTPEEVEATG    50
AQIILGNTFH LWLRPGQEIM KLHGDLHDFM QWKGPILTDS GGFQVFSLGD 100
IRKITEQGVH FRNPINGDPI FLDPEKSMEI QYDLGSDIVM IFDECTPYPA 150
DWDYAKRSME MSLRWAKRSR ERFDSLGNKN ALFGIIQGSV YEDLRDISVK 200
GLVDIGFDGY AVGGLAVGEP KADMHRILEH VCPQIPADKP RYLMGVGKPE 250
DLVEGVRRGI DMFDCVMPTR NARNGHLFVT DGVVKIRNAK YKSDTGPLDP 300
ECDCYTCRNY SRAYLHHLDR CNEILGARLN TIHNLRYYQR LMAGLRKAIE 350
EGKLESFVTD FYQRQGREVP PLNVD 375
Length:375
Mass (Da):42,594
Last modified:April 29, 2008 - v1
Checksum:i40271CA08D8A8820
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000970 Genomic DNA. Translation: ACB16174.1.
RefSeqiYP_001742541.1. NC_010498.1.

Genome annotation databases

EnsemblBacteriaiACB16174; ACB16174; EcSMS35_0438.
GeneIDi6146420.
KEGGiecm:EcSMS35_0438.
PATRICi18429719. VBIEscCol6161_0611.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000970 Genomic DNA. Translation: ACB16174.1 .
RefSeqi YP_001742541.1. NC_010498.1.

3D structure databases

ProteinModelPortali B1LJF4.
SMRi B1LJF4. Positions 3-364.
ModBasei Search...

Protein-protein interaction databases

STRINGi 439855.EcSMS35_0438.

Proteomic databases

PRIDEi B1LJF4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB16174 ; ACB16174 ; EcSMS35_0438 .
GeneIDi 6146420.
KEGGi ecm:EcSMS35_0438.
PATRICi 18429719. VBIEscCol6161_0611.

Phylogenomic databases

eggNOGi COG0343.
HOGENOMi HOG000223473.
KOi K00773.
OMAi FGSDIIM.
OrthoDBi EOG6SNDVG.

Enzyme and pathway databases

UniPathwayi UPA00392 .
BioCyci ECOL439855:GHHB-436-MONOMER.

Family and domain databases

Gene3Di 3.20.20.105. 1 hit.
HAMAPi MF_00168. Q_tRNA_Tgt.
InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view ]
PANTHERi PTHR11962. PTHR11962. 1 hit.
Pfami PF01702. TGT. 1 hit.
[Graphical view ]
SUPFAMi SSF51713. SSF51713. 1 hit.
TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5."
    Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R.
    J. Bacteriol. 190:6779-6794(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SMS-3-5 / SECEC.

Entry informationi

Entry nameiTGT_ECOSM
AccessioniPrimary (citable) accession number: B1LJF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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