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Reviewed, UniProtKB/Swiss-Prot B1LIM9 (BGAL_ECOSM)

Last modified November 3, 2009. Version 9. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Beta-galactosidase
      Short name=Beta-gal
    EC=3.2.1.23
Alternative name(s):
    Lactase
Gene names
Name: lacZ
Ordered Locus Names: EcSMS35_0375
OrganismEscherichia coli (strain SMS-3-5 / SECEC) [Complete proteome] [HAMAP]
Taxonomic identifier439855 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length1024 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity.

Binds 1 sodium ion per monomer By similarity.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10241024Beta-galactosidase HAMAP MF_01687
PRO_0000366990

Regions

Region538 – 5414Substrate binding By similarity

Sites

Active site4621Proton donor By similarity
Active site5381Nucleophile By similarity
Metal binding2021Sodium By similarity
Metal binding4171Magnesium 1 By similarity
Metal binding4191Magnesium 1 By similarity
Metal binding4621Magnesium 1 By similarity
Metal binding5981Magnesium 2 By similarity
Metal binding6021Sodium; via carbonyl oxygen By similarity
Metal binding6051Sodium By similarity
Binding site1031Substrate By similarity
Binding site2021Substrate By similarity
Binding site4621Substrate By similarity
Binding site6051Substrate By similarity
Binding site10001Substrate By similarity
Site3581Transition state stabilizer By similarity
Site3921Transition state stabilizer By similarity

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Sequences

Sequence LengthMass (Da)Tools
B1LIM9-1 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: A600DCF909D6DBFF

FASTA1,024116,542
        10         20         30         40         50         60 
MTMITDSLAV VLQRRDWENP GVTQLNRLAA HPHFASWRNS EEARTDRPSQ QLRSLNGEWR 

        70         80         90        100        110        120 
FAWFPAPEAV PESWLDCDLP DADTVVVPSN WQMHGYDAPI YTNVTYPITV NPPFVPAENP 

       130        140        150        160        170        180 
TGCYSLTFNI DECWLQKGQT RIIFDGVNSA FHLWCNGRWV GYGQDSRLPS EFDLSAFLRA 

       190        200        210        220        230        240 
GKNRLAVMVL RWSDGSYLED QDMWRMSGIF RDVSLLHKPT TQISDFHVAT RFNDDFSRAV 

       250        260        270        280        290        300 
LEAEVQMCGE LRDELRVTVS LWQGETQVAS GTTPFGGEII DERGGYADRV TLRLNVENPA 

       310        320        330        340        350        360 
LWSAEIPNLY RAVVELHTAD GTLIEAEACD VGFREVRIEN GLLLLNGKPV LIRGVNRHEH 

       370        380        390        400        410        420 
HPLHGQVMDE QTMVQDILLM KQNNFNAVRC SHYPNHPLWY TLCDRYGLYV VDEANIETHG 

       430        440        450        460        470        480 
MVPMNRLTDD PRWLPAMSER VTRMVQRDRN HPSVIIWSLG NESGHGANHD ALYRWIKSVD 

       490        500        510        520        530        540 
PSRPVQYEGG GADTTATDII CPMYARVDED QPFPAVPKWS IKKWLSLPGE LRPLILCEYA 

       550        560        570        580        590        600 
HAMGNSLGGF AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD ENGNPWSAYG GDFGDTPNDR 

       610        620        630        640        650        660 
QFCMNGLVFA DRTPHPALTE AKHQQQFFQF RLSGRTIEVT SEYLFRHSDN ELLHWSVALD 

       670        680        690        700        710        720 
GKPLASGEMP LDVAPQDKQL IELPELPQPE STGQLWLTVH VVQPNATAWS EAGHISAWQQ 

       730        740        750        760        770        780 
WRLAENLSVA LPSAPHAIPQ LTTSEMDFCI ELGNKRWQFN RQSGFLSQMW IGDEKQLLTP 

       790        800        810        820        830        840 
LRDQFIRAPL DNDIGVSEAT RIDPNAWVER WKAAGHYQAE VALLQCTADI LADAVLITTA 

       850        860        870        880        890        900 
HAWQHQGKTL FISRKTYRID GSGQMAITVD VEVASDTPHP ARIGLTCQLA QVAERVNWLG 

       910        920        930        940        950        960 
LGPQENYPDR LTAACFDRWD LPLSDMYTPY VFPSENGLRC GTRELNYGPH QWRGDFQFNI 

       970        980        990       1000       1010       1020 
SRYSQQQLME TSHRHLLHAE EGTWLNIDGF HMGIGGDDSW SPSVSAEFQL SAGRYHYQLV 


WCQK

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References

[1]"Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5."
Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R.
J. Bacteriol. 190:6779-6794(2008) [PubMed: 18708504] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000970 Genomic DNA. Translation: ACB17296.1.
RefSeqYP_001742478.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID6144942.
GenomeReviewsGene locus EcSMS35_0375 in contig CP000970_GR.
KEGGecm:EcSMS35_0375.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMANELLHWM.

Family and domain databases

HAMAPMF_01687.
[Tree]
InterProIPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006104. Glyco_hydro_2_carb-bd.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006103. Glyco_hydro_2_TIM.
IPR004199. Glyco_hydro_42_D5.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits.
G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameBGAL_ECOSM
AccessionPrimary (citable) accession number: B1LIM9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: November 3, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents