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Protein

Oxygen-dependent choline dehydrogenase

Gene

betA

Organism
Escherichia coli (strain SMS-3-5 / SECEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine at the same rate.UniRule annotation

Catalytic activityi

Choline + acceptor = betaine aldehyde + reduced acceptor.UniRule annotation
Betaine aldehyde + NAD+ + H2O = betaine + NADH.UniRule annotation

Cofactori

FADUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei473 – 4731UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi4 – 3330FADUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. betaine-aldehyde dehydrogenase activity Source: UniProtKB-EC
  2. choline dehydrogenase activity Source: UniProtKB-HAMAP
  3. flavin adenine dinucleotide binding Source: InterPro

GO - Biological processi

  1. glycine betaine biosynthetic process from choline Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciECOL439855:GHHB-340-MONOMER.
UniPathwayiUPA00529; UER00385.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-dependent choline dehydrogenaseUniRule annotation (EC:1.1.99.1UniRule annotation)
Short name:
CDHUniRule annotation
Short name:
CHDUniRule annotation
Alternative name(s):
Betaine aldehyde dehydrogenaseUniRule annotation (EC:1.2.1.8UniRule annotation)
Short name:
BADHUniRule annotation
Gene namesi
Name:betAUniRule annotation
Ordered Locus Names:EcSMS35_0342
OrganismiEscherichia coli (strain SMS-3-5 / SECEC)
Taxonomic identifieri439855 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000007011: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 562562Oxygen-dependent choline dehydrogenasePRO_1000133332Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi439855.EcSMS35_0342.

Structurei

3D structure databases

ProteinModelPortaliB1LIJ7.
SMRiB1LIJ7. Positions 1-531.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GMC oxidoreductase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2303.
HOGENOMiHOG000139600.
KOiK00108.
OMAiPRIGAEW.
OrthoDBiEOG67HJQP.

Family and domain databases

HAMAPiMF_00750. Choline_dehydrogen.
InterProiIPR011533. BetA.
IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view]
PfamiPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMsiTIGR01810. betA. 1 hit.
PROSITEiPS00623. GMC_OXRED_1. 1 hit.
PS00624. GMC_OXRED_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1LIJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFDYIIIGA GSAGNVLATR LTEDPNTTVL LLEAGGPDYR FDFRTQMPAA
60 70 80 90 100
LAFPLQGKRY NWAYETEPEP FMNNRRMECG RGKGLGGSSL INGMCYIRGN
110 120 130 140 150
ALDLDNWAQE PGLENWSYLD CLPYYRKAET RDVGENDYHG GDGPVSVTTS
160 170 180 190 200
KPGVNPLFEA MIEAGVQAGY PRTDDLNGYQ QEGFGPMDRT VTPHGRRAST
210 220 230 240 250
ARGYLDQAKS RSNLTIRTHA MTDHIIFDGK RAVGVEWLEG DSTIPTRAAA
260 270 280 290 300
NKEVLLCAGA IASPQILQRS GVGNAGLLAE FDIPLVHELP GVGENLQDHL
310 320 330 340 350
EMYLQYECKE PVSLYPALQW WNQPKIGAEW LFGGTGIGAS NHFEAGGFIR
360 370 380 390 400
SREEFAWPNI QYHFLPVAIN YNGSNAVKEH GFQCHVGSMR SPSRGHVRIK
410 420 430 440 450
SRAPHQHPAI LFNYMSHEQD WQEFRDAIRI TREIMHQPAL DQYHGREISP
460 470 480 490 500
GVECQTDEQL DEFVRNHAET AFHPCGTCKM GYDEMSVVDG EGRVHGLEGL
510 520 530 540 550
RVVDASIMPQ IITGNLNATT IMIGEKMADM IRGKDALPRS TAGYYVANGM
560
PVRAKKMSRD VN
Length:562
Mass (Da):62,430
Last modified:April 29, 2008 - v1
Checksum:iB80FCC35FC3C9A1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000970 Genomic DNA. Translation: ACB17123.1.
RefSeqiYP_001742446.1. NC_010498.1.

Genome annotation databases

EnsemblBacteriaiACB17123; ACB17123; EcSMS35_0342.
GeneIDi6146020.
KEGGiecm:EcSMS35_0342.
PATRICi18429523. VBIEscCol6161_0514.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000970 Genomic DNA. Translation: ACB17123.1.
RefSeqiYP_001742446.1. NC_010498.1.

3D structure databases

ProteinModelPortaliB1LIJ7.
SMRiB1LIJ7. Positions 1-531.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi439855.EcSMS35_0342.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB17123; ACB17123; EcSMS35_0342.
GeneIDi6146020.
KEGGiecm:EcSMS35_0342.
PATRICi18429523. VBIEscCol6161_0514.

Phylogenomic databases

eggNOGiCOG2303.
HOGENOMiHOG000139600.
KOiK00108.
OMAiPRIGAEW.
OrthoDBiEOG67HJQP.

Enzyme and pathway databases

UniPathwayiUPA00529; UER00385.
BioCyciECOL439855:GHHB-340-MONOMER.

Family and domain databases

HAMAPiMF_00750. Choline_dehydrogen.
InterProiIPR011533. BetA.
IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view]
PfamiPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMsiTIGR01810. betA. 1 hit.
PROSITEiPS00623. GMC_OXRED_1. 1 hit.
PS00624. GMC_OXRED_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5."
    Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R.
    J. Bacteriol. 190:6779-6794(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SMS-3-5 / SECEC.

Entry informationi

Entry nameiBETA_ECOSM
AccessioniPrimary (citable) accession number: B1LIJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: January 7, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.