Reviewed, UniProtKB/Swiss-Prot B1LI85 (GLPE_ECOSM)

Names and origin

Protein names

Recommended name:
    Thiosulfate sulfurtransferase glpE
    EC=2.8.1.1

Gene names

Name:	glpE
Ordered Locus Names:	EcSMS35_3705

Organism

Escherichia coli (strain SMS-3-5 / SECEC) [Complete proteome] [HAMAP]

Taxonomic identifier

439855 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide By similarity.
Catalytic activity	Thiosulfate + cyanide = sulfite + thiocyanate. HAMAP MF_01009
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glpE family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiosulfate sulfurtransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 108	108	Thiosulfate sulfurtransferase glpE HAMAP MF_01009		PRO_1000190098
Regions
	Domain	17 – 105	89	Rhodanese
Sites
	Active site	65	1	Cysteine persulfide intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1LI85-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 03C72DEAC2A8F016	FASTA	108	12,054
	10 20 30 40 50 60 MDQFECINVA DAHQKLQEKE AVLVDIRDPQ SFAMGHAAQA FHLTNDTLGA FMRDNDFDTP 70 80 90 100 VMVMCYHGNS SKGAAQYLLQ QGYDVVYSID GGFEAWQRQF PAEVAYGA

References

[1]

"Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5." Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R. J. Bacteriol. 190:6779-6794(2008) [PubMed: 18708504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000970 Genomic DNA. Translation: ACB20128.1.
RefSeq	YP_001745672.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6142990.
GenomeReviews	Gene locus EcSMS35_3705 in contig CP000970_GR.
KEGG	ecm:EcSMS35_3705.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FSAWHEA.
Family and domain databases
HAMAP	MF_01009. [Tree]
InterPro	IPR001763. Rhodanese-like. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 1 hit.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
SMART	SM00450. RHOD. 1 hit. [Graphical view]
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPE_ECOSM

Accession

Primary (citable) accession number: B1LI85

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)