Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Escherichia coli (strain SMS-3-5 / SECEC)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei198 – 1981ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL439855:GHHB-3656-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotationSAAS annotation (EC:6.1.1.2UniRule annotationSAAS annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:trpSUniRule annotationImported
Ordered Locus Names:EcSMS35_3660Imported
OrganismiEscherichia coli (strain SMS-3-5 / SECEC)Imported
Taxonomic identifieri439855 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000007011 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1LHH9.
SMRiB1LHH9. Positions 4-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 209"HIGH" regionUniRule annotation
Motifi195 – 1995"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000059940.
KOiK01867.
OMAiERFKNKG.
OrthoDBiEOG686NJQ.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1LHH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKPIVFSGA QPSGELTIGN YMGALRQWVN MQDDYHCIYC IVDQHAITVR
60 70 80 90 100
QDAQKLRKAT LDTLALYLAC GIDPEKSTIF VQSHVPEHAQ LGWALNCYTY
110 120 130 140 150
FGELSRMTQF KDKSARYAEN INAGLFDYPV LMAADILLYQ TNLVPVGEDQ
160 170 180 190 200
KQHLELSRDI AQRFNALYGD IFKVPEPFIP KSGARVMSLL EPTKKMSKSD
210 220 230 240 250
DNRNNVIGLL EDPKSVVKKI KRAVTDSDEP PVVRYDVQNK AGVSNLLDIL
260 270 280 290 300
SAVTGQSIPE LEKQFEGKMY GHLKGEVADA VSGMLTELQE RYHRFRNDEA
310 320 330
FLQQVMKDGA EKASAHASRT LKAVYEAIGF VAKP
Length:334
Mass (Da):37,424
Last modified:April 29, 2008 - v1
Checksum:iDEB972FAFDAF3E28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000970 Genomic DNA. Translation: ACB16982.1.
RefSeqiWP_000165543.1. NC_010498.1.

Genome annotation databases

EnsemblBacteriaiACB16982; ACB16982; EcSMS35_3660.
KEGGiecm:EcSMS35_3660.
PATRICi18436251. VBIEscCol6161_3809.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000970 Genomic DNA. Translation: ACB16982.1.
RefSeqiWP_000165543.1. NC_010498.1.

3D structure databases

ProteinModelPortaliB1LHH9.
SMRiB1LHH9. Positions 4-332.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB16982; ACB16982; EcSMS35_3660.
KEGGiecm:EcSMS35_3660.
PATRICi18436251. VBIEscCol6161_3809.

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000059940.
KOiK01867.
OMAiERFKNKG.
OrthoDBiEOG686NJQ.

Enzyme and pathway databases

BioCyciECOL439855:GHHB-3656-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5."
    Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R.
    J. Bacteriol. 190:6779-6794(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SMS-3-5 / SECECImported.

Entry informationi

Entry nameiB1LHH9_ECOSM
AccessioniPrimary (citable) accession number: B1LHH9
Entry historyi
Integrated into UniProtKB/TrEMBL: April 29, 2008
Last sequence update: April 29, 2008
Last modified: July 22, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.