B1LGE7 (RNB_ECOSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exoribonuclease 2
EC=3.1.13.1

Alternative name(s):
Exoribonuclease II
Short name=RNase II
Short name=Ribonuclease II

Gene names

Name:	rnb
Ordered Locus Names:	EcSMS35_1841

Organism

Escherichia coli (strain SMS-3-5 / SECEC) [Complete proteome] [HAMAP]

Taxonomic identifier

439855 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	644 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3' to 5' direction By similarity. HAMAP-Rule MF_01036
Catalytic activity	Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates. HAMAP-Rule MF_01036
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the RNR ribonuclease family. RNase II subfamily. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA catabolic process Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW exoribonuclease II activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 644	644	Exoribonuclease 2 HAMAP-Rule MF_01036		PRO_1000135868
Regions
	Domain	561 – 643	83	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	B1LGE7 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 36B16704BC964394	FASTA	644	72,463
	10 20 30 40 50 60 MFQDNPLLAQ LKQQLHSQTP RAEGVVKATE KGFGFLEVDA QKSYFIPPPQ MKKVMHGDRI 70 80 90 100 110 120 IAVIHSEKER ESAEPEELVE PFLTRFVGKV QGKNDRLAIV PDHPLLKDAI PCRAARGLNH 130 140 150 160 170 180 EFKEGDWAVA EMRRHPLKGD RSFYAELTQY ITFGDDHFVP WWVTLARHNL EKEAPDGVAT 190 200 210 220 230 240 EMLDEGLVRE DLTALDFVTI DSASTEDMDD ALFAKALPDD KLQLIVAIAD PTAWIAEGSK 250 260 270 280 290 300 LDKAAKIRAF TNYLPGFNIP MLPRELSDDL CSLRANEVRP VLACRMTLSA DGTIEDNIEF 310 320 330 340 350 360 FAATIESKAK LVYDQVSDWL ENTGDWQPES EAIAEQVRLL AQICQRRGEW RHNHALVFKD 370 380 390 400 410 420 RPDYRFILGE KGEVLDIVAE PRRIANRIVE EAMIAANICA ARVLRDKLGF GIYNVHMGFD 430 440 450 460 470 480 PANADALAAL LKTHGLHVDA EEVLTLDGFC KLRRELDAQP TGFLDSRIRR FQSFAEISTE 490 500 510 520 530 540 PGPHFGLGLE AYATWTSPIR KYGDMINHRL LKAVIKGETA TRPQDEITVQ MAERRRLNRM 550 560 570 580 590 600 AERDVGDWLY ARFLKDKAGT DTRFAAEIVD ISRGGMRVRL VDNGAIAFIP APFLHAVRDE 610 620 630 640 LVCSQENGTV QIKGETAYKV TDVIDVTIAE VRMETRSIIA RPVA

References

[1]

"Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5." Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R. J. Bacteriol. 190:6779-6794(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SMS-3-5 / SECEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000970 Genomic DNA. Translation: ACB17629.1.
RefSeq	YP_001743895.1. NC_010498.1.
3D structure databases
ProteinModelPortal	B1LGE7.
SMR	B1LGE7. Positions 5-644.
ModBase	Search...
Protein-protein interaction databases
STRING	439855.EcSMS35_1841.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB17629; ACB17629; EcSMS35_1841.
GeneID	6147184.
KEGG	ecm:EcSMS35_1841.
PATRIC	18432555. VBIEscCol6161_2000.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4776.
HOGENOM	HOG000271427.
KO	K01147.
OMA	ERDIADW.
ProtClustDB	PRK05054.
Enzyme and pathway databases
BioCyc	ECOL439855:GHHB-2191-MONOMER.
Family and domain databases
HAMAP	MF_01036. RNase_II.
InterPro	IPR011129. Cold_shock_prot. IPR012340. NA-bd_OB-fold. IPR003029. Rbsml_prot_S1_RNA-bd_dom. IPR022967. RNA-binding_domain_S1. IPR013223. RNase_B_OB_dom. IPR011804. RNase_II. IPR001900. RNase_II/R. IPR022966. RNase_II/R_CS. IPR004476. RNase_II/RNase_R. [Graphical view]
PANTHER	PTHR23355:SF6. PTHR23355:SF6. 1 hit.
Pfam	PF08206. OB_RNB. 1 hit. PF00773. RNB. 1 hit. PF00575. S1. 1 hit. [Graphical view]
SMART	SM00357. CSP. 1 hit. SM00955. RNB. 1 hit. SM00316. S1. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00358. 3_prime_RNase. 1 hit. TIGR02062. RNase_B. 1 hit.
PROSITE	PS01175. RIBONUCLEASE_II. 1 hit. PS50126. S1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNB_ECOSM

Accession

Primary (citable) accession number: B1LGE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program