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Protein

3-dehydroquinate dehydratase

Gene

aroD

Organism
Escherichia coli (strain SMS-3-5 / SECEC)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.UniRule annotation

Catalytic activityi

3-dehydroquinate = 3-dehydroshikimate + H2O.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroF)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE), Quinate/shikimate dehydrogenase (ydiB)
  5. Shikimate kinase 1 (aroK), Shikimate kinase 2 (aroL)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei213-dehydroquinateUniRule annotation1
Binding sitei823-dehydroquinateUniRule annotation1
Active sitei143Proton donor/acceptorUniRule annotation1
Active sitei170Schiff-base intermediate with substrateUniRule annotation1
Binding sitei2133-dehydroquinateUniRule annotation1
Binding sitei2323-dehydroquinateUniRule annotation1
Binding sitei2363-dehydroquinateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandSchiff base

Enzyme and pathway databases

UniPathwayiUPA00053; UER00086

Names & Taxonomyi

Protein namesi
Recommended name:
3-dehydroquinate dehydrataseUniRule annotation (EC:4.2.1.10UniRule annotation)
Short name:
3-dehydroquinaseUniRule annotation
Alternative name(s):
Type I DHQaseUniRule annotation
Type I dehydroquinaseUniRule annotation
Short name:
DHQ1UniRule annotation
Gene namesi
Name:aroDUniRule annotation
Ordered Locus Names:EcSMS35_1503
OrganismiEscherichia coli (strain SMS-3-5 / SECEC)
Taxonomic identifieri439855 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000007011 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001895681 – 2523-dehydroquinate dehydrataseAdd BLAST252

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1LE43
SMRiB1LE43
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni46 – 483-dehydroquinate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the type-I 3-dehydroquinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000105514
KOiK03785
OMAiATMAMGE

Family and domain databases

CDDicd00502 DHQase_I, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00214 AroD, 1 hit
InterProiView protein in InterPro
IPR018508 3-dehydroquinate_DH_AS
IPR013785 Aldolase_TIM
IPR001381 DHquinase_I
PfamiView protein in Pfam
PF01487 DHquinase_I, 1 hit
TIGRFAMsiTIGR01093 aroD, 1 hit
PROSITEiView protein in PROSITE
PS01028 DEHYDROQUINASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B1LE43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTVTVKDLV IGTGAPKIIV SLMAKDIASV KSEALAYREA DFDILEWRVD
60 70 80 90 100
HYADLSNVES VMAAAKILRE TMPEKPLLFT FRSAKEGGEQ AISTEAYIAL
110 120 130 140 150
NRAAIDSGLV DMIDLELFTG DDQVKETVAY AHAHDVKVVM SNHDFHKTPE
160 170 180 190 200
AEEIIARLRK MQSFDADIPK IALMPQSTSD VLTLLAATLE MQEQYADRPI
210 220 230 240 250
ITMSMAKTGV ISRLAGEVFG SAATFGAVKK ASAPGQISVN DLRTVLTILH

QA
Length:252
Mass (Da):27,467
Last modified:April 29, 2008 - v1
Checksum:i0D4E7FAEAD5FCD48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000970 Genomic DNA Translation: ACB16389.1
RefSeqiWP_000860201.1, NC_010498.1

Genome annotation databases

EnsemblBacteriaiACB16389; ACB16389; EcSMS35_1503
KEGGiecm:EcSMS35_1503

Similar proteinsi

Entry informationi

Entry nameiAROD_ECOSM
AccessioniPrimary (citable) accession number: B1LE43
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: March 28, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health