B1LDZ1 (NADE_ECOSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	EcSMS35_1451

Organism

Escherichia coli (strain SMS-3-5 / SECEC) [Complete proteome] [HAMAP]

Taxonomic identifier

439855 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000118621
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1LDZ1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 359600D4A2DF4583	FASTA	275	30,653
	10 20 30 40 50 60 MTLQQQIIKA LGAKPQINAE EEIRRSVDFL KSYLQTYPFI KSLVLGISGG QDSTLAGKLC 70 80 90 100 110 120 QMAINELRQE TGNESLQFIA VRLPYGVQAD EQDCQDAIAF IQPDRVLTVN IKGAVLASEQ 130 140 150 160 170 180 ALREAGIELS DFVRGNEKAR ERMKAQYSIA GMTSGVVVGT DHAAEAITGF FTKYGDGGTD 190 200 210 220 230 240 INPLYRLNKR QGKQLLAALG CPEHLYKKAP TADLEDDRPS LPDEVALGVT YDNIDDYLEG 250 260 270 KNLPEQVART IENWYLKTEH KRRPPITVFD DFWKK

References

[1]

"Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5." Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C., Ravel J., Stepanauskas R. J. Bacteriol. 190:6779-6794(2008) [PubMed: 18708504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SMS-3-5 / SECEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000970 Genomic DNA. Translation: ACB16374.1.
RefSeq	YP_001743509.1. NC_010498.1.
3D structure databases
ProteinModelPortal	B1LDZ1.
SMR	B1LDZ1. Positions 2-275.
ModBase	Search...
Protein-protein interaction databases
STRING	B1LDZ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000064037; EBESCP00000061679; EBESCG00000063084.
GeneID	6143207.
GenomeReviews	Gene locus EcSMS35_1451 in contig CP000970_GR.
KEGG	ecm:EcSMS35_1451.
PATRIC	18431771. VBIEscCol6161_1610.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009560.
HOGENOM	HBG351567.
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	ECOL439855:ECSMS35_1451-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_ECOSM

Accession

Primary (citable) accession number: B1LDZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program