Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Thermotoga sp. (strain RQ2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei45Thioimide intermediateUniRule annotation1
Active sitei52Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processQueuosine biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:TRQ2_0136
OrganismiThermotoga sp. (strain RQ2)
Taxonomic identifieri126740 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000001687 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001343151 – 137NADPH-dependent 7-cyano-7-deazaguanine reductaseAdd BLAST137

Structurei

3D structure databases

ProteinModelPortaliB1LCN9.
SMRiB1LCN9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 70Substrate bindingUniRule annotation3
Regioni87 – 88Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000009528.
KOiK09457.
OMAiLYINSYR.
OrthoDBiPOG091H01A3.

Family and domain databases

HAMAPiMF_00818. QueF_type1. 1 hit.
InterProiView protein in InterPro
IPR029500. QueF.
IPR016856. QueF_type1.
PfamiView protein in Pfam
PF14489. QueF. 1 hit.
PIRSFiPIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMsiTIGR03139. QueF-II. 1 hit.

Sequencei

Sequence statusi: Complete.

B1LCN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKAEGRIFD FKGHDAIRTD FLEAIDFDGK DEYIKIETDE FSAVCPFSGL
60 70 80 90 100
PDIGRVIIEY YPDGGKIVEL KSLKYYFVSF RNVGIYQEEA TKRIYEDLKN
110 120 130
LLKTDRIRVT VIYNIRGGIK TTTQMGSLEG KKSGEVE
Length:137
Mass (Da):15,675
Last modified:April 29, 2008 - v1
Checksum:i597073A219ECF27C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000969 Genomic DNA. Translation: ACB08497.1.
RefSeqiWP_004080885.1. NC_010483.1.

Genome annotation databases

EnsemblBacteriaiACB08497; ACB08497; TRQ2_0136.
GeneIDi29653517.
KEGGitrq:TRQ2_0136.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiQUEF_THESQ
AccessioniPrimary (citable) accession number: B1LCN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: June 7, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families